Sonidel Limited
Non Viral Sonoporation and Electroporation Gene Transfection Methodologies
Organoid Request Trial
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Institute/Organisation
Name
*
First
Last
Email
*
Phone Number
Organoid model type
CRC (colorectal cancer)
PDAC (pancreatic ductal adenocarcinoma)
Brain / Cortical
Lung
Kidney
Liver
Other
If other - please describe application
*
Average Organoid Diameter (µm range)
<150 µm (small / cystic stage)
150–300 µm
300–600 µm
>600 µm (mature / dense)
Estimated Lumen Accessibility
Open / easy to access
Partially accessible
Thick / difficult access
Delivery Cargo Type
CRISPR RNP (Cas9 protein + sgRNA)
Plasmid DNA (<8 kb)
Large plasmid (>8–10 kb)
mRNA
siRNA / shRNA
CRISPR donor template (HDR)
Construct Size (kb)
Experimental goal
Rapid transient expression (3–7 days)
CRISPR knockout (KO, NHEJ),
Knock-in / HDR
Reporter line generation
Functional pathway screening
Pre-viral pilot validation
Other
Other Goal
Current Workflow & Constraints
We currently use viral transduction
We do not have BSL-2 viral capability
We want to avoid integration footprint
We need high viability (>70%)
Working with patient-derived PDOs
Integrating into organoid-on-chip system
Other Workflow/Constraints
Organoid on chip
No
Yes
Optional: Anything else we should know? (ECM type, Matrigel %, passage number, fragility concerns)
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Pages
Chick and Quail Embryo (in ovo) – Neural Tube, Mesencephalon, Diencephalon (HH* Stage 10
Single-Cell/Micro-Particle_Transfer-Micro_Pick_and_Place_(MPP)_System
0.2 Organoid Landing Page
1. Test Colon Organoid
10.Test CRC and PDAC Organoid Labs
2. Test Brain Organoid
2. Test Brain Organoid – do not use
3. Test Organoid on a Chip
3T3-L1 – Mouse Preadipocytes – In Adherence
4. Test DevBio Labs
5. Test Disease Modelling Organoids Labs
6. Test Core Facility Organoid
7. Test PDO Biobanks
8. Test Single Cell Organoid
9. CRISPR Labs
AcquaStain
Adherent Cells
Adherent Electroporation Applications
Adult Mouse and Rat (In vivo) – Bladder
Adult Mouse and Rat (In Vivo) – Brain
Adult Mouse and Rat (In vivo) – Kidney
Adult Mouse and Rat (In vivo) – Knee Cartilage
Adult Mouse and Rat (In vivo) – Liver
Adult Mouse and Rat (In vivo) – Synovial Membrane
Adult Mouse and Rat (In vivo) – Testis, Ovary
Adult Mouse and Rat (In vivo) – Vessel
Adult Mouse, Rat, Rabbit and Dog (In Vivo) – Skin (Epidermus, Dermus, Hypodermus)
Adult Mouse, Rat, Rabbit, Dog and Monkey (DNA Vaccine)
ag teacht abhaile200219992*s
Amaxa_Nucleofector_II_Pulse_data_with_NEPA21_Monitoring_System
Application Support Page – NEPA21
Bee (In Vivo)
Block – coloured
Bovine Cloning – LF101/LF201
Cancer Therapy & CUY21
Cat Cloning – LF101/LF201
Chick Embryo (New Culture) – Gastrulation, Neural Plate (HH Stage 3-7)
Chick Embryo (Organ Culture) – Digestive Organ Epithelial Cell (HH Stage 3-7)
Chip readiness decision checklist
Cleaning Adherent Electrodes
CLICK Method
Compare CUY21 System with Competing Devices
Comparison of the CUY21SC and BTX ECM830 Square Waves at various voltages
Comparison of the CUY21SC and BTX ECM830 Square Waves at 100V
Comparison of the CUY21SC and BTX ECM830 Square Waves at 15V
Comparison of the CUY21SC and BTX ECM830 Square Waves at 20V
Comparison of the CUY21SC and BTX ECM830 Square Waves at 25V
Comparison of the CUY21SC and BTX ECM830 Square Waves at 30V
Comparison of the CUY21SC and BTX ECM830 Square Waves at 40V
Comparison of the CUY21SC and BTX ECM830 Square Waves at 50V
Connector Cables for NEPA21 / CUY21 Electroporator and Electrodes
Contact Us
Controlling Electrofusion – LF101 & LF201
CU700 and CU710 Electroporation and Cell Fusion Monitoring Systems
Cultured Mouse Embryos and Large Tissue
Cultured Neurons – Sonoporation
Current Agrobacterium & Microinjection Methods – Disadvantages
Cuvette Chamber (CU500) and Cuvette Stand Holder (CU600)
Cuvette Pricing
CUY21 Publication List, Electrode Recommendations and Protocol Information
CUY21SC – Square Wave Electroporator
CUY21SC Applications and Electrode Recommendations
CUY21SC Applications and Electrode Recommendations
CUY900-13-3-5_Technical_Drawings
Demonstration Request
Direct gene transfer into mature plant seeds using electrical pulses and supersonic treatment
Dog Cloning – LF101/LF201
ECFG21 3 Step Process
ECFG21-LF201-LF101 Illustrated Applications
ECFG21_Electrofusion_Optimisation_for_Hybridoma_Production_for_Monoclonal_Antibody_pdf
Electro Cell Fusion
ECFG21 Super Electro-Cell Fusion Generator for hybridoma production and nuclear transfer
Electrodes
Electroporation
Improve Electroporation Transfection Efficiency with the CU902 Polarity Exchanger
Maximise Electroporation Transfection Efficiency and Reproducibility with the CUY21SC Electroporation System and CU902 Polarity Exchanger
NEPA Electroporation Cuvettes
NEPA21 Electroporator
#2584 (no title)
1321N1 – Human Astrocytoma cells
293T (HEK293T) Human Embryonic Kidney Cells
3T3-L1 – Mouse Preadipocytes
4T1 – Mouse Breast Cancer cells
A172-Human Glioblastoma cells
BFF – Bovine Fetal Fibroblasts
BMBa – Mouse Bone marrow-derived basophils
C2C12 – Mouse Myoblast cells
C6 – Rat Glioma cells – In Adherence
Ca9-22 – Human Squamous Carcinoma cells
Cell Lines – TIG-3 – Human Embryonic Lung Fibroblasts
Cell Lines-3T3-L1 – Mouse Preadipocytes – In Adherence
Cell Lines-ATDC5 – Mouse Pre-chondrocytic cells
Cell Lines-BT-20 – Human Breast Cancer cells
Cell Lines-BV-2 – Mouse Microglial cells
Cell Lines-C6-rat Glioma Cells
Cell Lines-CCD1079sk-Human Skin Fibroblasts
Cell Lines-CHO-Chinese Hamster Ovary Cells
Cell Lines-CHO-K1-Chinese Hamster Ovary Cells
Cell Lines-DT40-Chicken B Cells
Cell Lines-EPC – Human Endothelial Progenitor Cell
Cell Lines-GTS9-Ginbuna Thymus Cells
Cell Lines-H69 – Human Small-Cell Lung Cancer cells
Cell Lines-H9c2-Rat Ventricular Myoblasts
Cell Lines-HaCaT-Human Keratinocyte Cells
Cell Lines-Hela-Human Cervical Carcinoma Cells
Cell Lines-HepG2-Human Hepatoma Cells
Cell Lines-HT1080-Human Fibrosarcoma Cells
Cell Lines-HTR-8-Svneo-Human Trophoblast Cells
Cell Lines-HuH-7 – Human Hepatoma cells
Cell Lines-KG-1-C-Human Oligodendroglial Cells
Cell Lines-KG-1-Human Acute Myeloid Leukemia cells
Cell Lines-KMST-6-Human Fibroblast
Cell Lines-L-Mouse Fibroblasts
Cell Lines-MC3T3-E1-Mouse Osteoblastic Cells
Cell Lines-MCF-10A – Human Breast Cells
Cell Lines-MIA-PaCa-2-Human Pancreatic Carcinoma Cells
Cell Lines-MIN6 – Mouse Pancreatic Islet Beta cells
Cell Lines-MIN6-Mouse Pancreatic Beta Cells
Cell Lines-MRC-5-Human Embryonic Lung Fibroblasts
Cell Lines-Mutu-Human Burkitt Lymphoma Cells
Cell Lines-Neurospheres – Neural Stem cells Derived from Mouse SVZ
Cell Lines-OVCAR-3-Human Ovarian Carcinoma Cells
Cell Lines-PC12-Rat Adrenal Pheochromocytoma Cells
Cell Lines-Primary – Mouse Liver cells
Cell Lines-Primary – Rat Cerebral Cortex Neurons
Cell Lines-Primary – Rat Meningeal Fibroblasts
Cell Lines-Primary – Rat Neurons – In Adherence
Cell Lines-Primary Rat Neurons – In Adherence – P7 rat hippocampus
Cell Lines-RAW264.7-Mouse Macrophage-like Cells
Cell Lines-REF-Rat Embryonic Fibroblasts
Cell Lines-RMG-1-Human Ovarian Clear Cell Adenocarcinoma
Cell Lines-SK-N-SH-Human Neuroblastoma Cells
Cell Lines-SUSM-1-Human Fibroblasts
Cell Lines-TE-1-Human Esophageal Carcinoma Cells
Cell Lines-TE-8-Human Esophageal Carcinoma Cells
Cell Lines-TIG-7-Human Embryonic Lung Fibroblasts
Cell Lines-TS-Mouse Trophoblast Stem Cells
Cell Lines-UT-7/TPO-Human Megakaryocytic cells
Cell Lines-XS106-Mouse Dendritic Cells
Cell-Lines-A6 – Xenopus Kidney Epithelial cells
Colon-26 – Mouse Colon Adenocarcinoma cells
Comparison_of_NEPA21_with_Amaxa-Nucleofector_Neon_and_Lipofectamine-2000.pdf
Comparison_of_NEPA21_with_Bio-Rad
HGF – Human Gingival Fibroblasts
HSC-3 – Human Squamous Carcinoma cells
HSC-4 – Human Squamous Carcinoma cells
In Vivo Intact Mouse Tumor Electroporation video with the NEPA21 Superelectroporator
Jurkat – Human T-cell Leukemia cells
K562 – Human Erythroleukemia cells
LCL – Human Lymphoblastoid cell
LLc1 (LL/2) – Mouse Lewis lung cell carcinoma 1 cells
MC/9 – Mouse Mast cells
MDCK – Madrin-Darby Canine
MEF – Mouse Embryonic Fibroblasts
Mouse ES cells
Mouse iPS cells
NEPA21_Algae-Transformation_without_cell-wall_removal
NEPA21_compared_with_BTX_ECM830_-_Measurement_and_Visual_Display_of_Delivered_EP_Pulse
NEPA21_Japanese_Language_Protocol_Information
Neuro-2a – Mouse Neuroblastoma cells
NIH-3T3 – Mouse Embryonic Fibroblasts
P19C6 – Mouse Embryonic Carcinoma cells
Primary – Chick Embryonic Cerebellar Granule Cells
Primary – Human Endometrial Stromal Cells
Primary – Human Mesenchymal Stem cells
Primary – Human T cells
Primary – Human Uterine Cervical Fibroblast
Primary – Mouse Cerebral Cortex Neurons
Primary – Mouse Embryonic Skin Fibroblasts
Primary – Mouse Glial cells-In Adherence
Primary – Mouse Hippocampal Neurons-In Adherence
Primary – Mouse Microglia cells
Primary – Mouse Neural Stem cells
Primary – Mouse Neurons-In Adherence
Primary – Rat Bulbar Neurons
Primary – Rat Meningeal Fibroblasts
Primary BMMC – Mouse Bone Marrow-Derived Mast Cells
Primary Chick Embryonic Fibroblasts
Primary HASM – Human Airway Smooth Muscle cells
Primary MEF – Mouse Embryonic Fibroblasts
Primary OEC – Rat Olfactory Ensheathing Cells
Ramos-BlueTM – Human Burkitt Lymphoma cells
Resistance_Ranges_for_NEPA21_Applications
RSC96 – Rat Schwann cells
SH-SY5Y – Human Neuroblastoma cells
SH-SY5Y – Human Neuroblastoma cells In Adherence
SKOV-3 – Human Ovarian Carcinoma cells
THP-1 – Human acute monocytic leukemia cells
TS – Mouse Trophoblast Stem Cells
TT2 – Mouse TT2 ES cells
TtT-GF – Rat Anterior Pituitary cells
WR19L – Mouse T-cell lymphoma cells
NEPA21, CUY21SC and CUY21EDIT Multiple Applications and Electrode Recommendations
Photo comparison of NEPA21 with CUY21EDIT
Video of Direct delivery of MIF Morpholinos into the Zebrafish Embryo using the NEPA21
Electroporation Protocols – In Vitro, In Ovo, In Utero, In Vivo, Ex Vivo and New Culture
ELEP021 Square Wave Electroporation
ELEPO21 Publications
ELEPO21 Results
ELEPO21 versus NEPA21
ELEPO21_for_Bacterial_Yeast_Fungi_Mammalian_Cell_EP
Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
Expression Plasmids Optimised for Ultrasound Mediated Gene Transfer
FH_Newsletter_Template
Fish – Egg
Free NEPA21 Demo and Trial :-Zygote Electroporation for Transgenic Animal Production
Gene Transfer and Expression using Ultrasound – Sonoporation
Gene transfer of Arabidopsis Seeds – pdf
Gene Transfer to Silk Worm Eggs – pdf
Gene Transfer to Yeast – pdf
General Sonoporation
How To Order
HSC-2 – Human Squamous Carcinoma cells
iGONAD Technique
In Ovo Electroporation
In Ovo Electroporation Resource
In Ovo Lim Bud – Sonoporation
In Utero Electrode Recommendation
In Utero Electroporation
In Utero Electroporation Video with NEAP21 SuperElectroporator
In Vitro Cell Transfection Database: NEPA21
Intestine-Gut Electroporation
In_Utero-Mouse_Embryo-Cortex-Cerebellum- E12.5
In_Utero-Mouse_Embryos-E13.5_with_CUY650P5
LF201 – Electro Cell Fusion Generator
LF201 Publication Listing for Cell Fusion and Nuclear Transfer
Low-cost solution to increase Electroporation Transfection Efficiency
LTM-1000 : Laser Thermal Microinjector
Meeting – NEPA21 overview
Micro Pick and Place (MPP) Videos
Microbubbles
Microbubbles for Sonoporation Gene Expression & Gene Transfection
SONIDEL MB101 and Adherent Cells in Opticell
SONIDEL MB101 and Cells Sonoporated in 3-D Martix
SONIDEL MB101 and Minicircle DNA MC07.CMV-Luc
SONIDEL MB101 and Plasmid DNA pCMV-Luc
Microfluidic Chamber
Mouse and Rat (Whole Embryo Culture) – Neural Plate Metencephalon, Somite (Mouse E7-13)
Mouse and Rat (Whole Embryo Culture) – Neural Tube (Mouse E9.5, Rat 11.5)
Mouse and Rat (Whole Embryo Culture) – Telencephalon (Mouse E7-13)
Mouse and Rat Small Tissue Electrode Recommendation and Protocol
Mouse and Rat Tissue and Brain Slice
Mouse Embryo (In Utero) – Cerebral Cortex, All Areas (>E13.5)
Mouse Embryo (In Utero) – Cerebral Cortex, All Areas (E12.5)
Mouse Embryo (In Utero) – Cerebral Cortex, Specific Areas (>E13.5)
Mouse Embryo (In Utero) – Forebrain Ventricle (E14)
Mouse Embryo (In Utero) – Spinal Cord (E10.5)
Mouse Embryo – Entire Brain
Mouse Embryo – Eye
Mouse Embryo – Spinal Cord
Mouse Embryo Cloning – LF101/LF201
Mouse/Rat – Skin
Mouse/Rat Adult – Brain (Cerebellum)
Mouse/Rat – Knee Joint
Mouse/Rat – Muscle
Mouse_Rat_In_Vivo_Spinal_Cord_Electroporation
MPP Electrode Holder
Muscle Wound
NEPA Porator
NEPA Porator for E Coli
NEPA21 – Cells in Adherence Results
NEPA21 / CUY21 Illustrated Applications
Chick embryo Electroporation using News culture gastrula
Direct Gene Transfer into Mature Seeds via Electroporation
Electrochemotherapy for digital chondrosarcoma
Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect
Electroporation for mammalian embryos in the whole embryo culture system
Electroporation of Xenopus embryo: Gene Delivery into the Primordium Eye at the Neural Plate Stage
Electroporation-mediated gene transfer in the adult rat brain
Electroporation-mediated gene transfer system applied to cultured CNS neurons
Gene transfer into embryonic brains using in utero electroporation technique
Gene transfer into muscle by In Vivo electroporation
Gene transfer into single cell by electroporation
In vivo gene transfer into the adult honeybee brain by using electroporation
Knock-down by transfection of shRNA expression vector by electroporation
Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)
Misexpression of the gene of interest by in ovo electroporation
[Plant Seed] Direct Gene Transfer into Mature Seeds via Electroporation
NEPA21 / CUY21 Publications by Research Interest
NEPA21 Cell Image Viability and Transfection Efficiency Results
NEPA21 Cell Transfection Data
NEPA21 Colon Organoid Workflows
NEPA21 CORE FACILITY WORKFLOW
NEPA21 CRC AND PDAC WORKFLOW
NEPA21 CRISPR LABS WORKFLOW
NEPA21 DISEASE MODELLING WORKFLOW
NEPA21 In Vitro Results
NEPA21 Neuroscience and Genome Editing Publications
NEPA21 Organoid Trial
NEPA21 PDO BIOBANKS WORKFLOW
NEPA21 Publication List
NEPA21 Results, Applications and Publications — NEW PRODUCTS
NEPA21 SINGLE CELL AND SPATIAL GENOMICS WORKFLOW
NEPA21 Workflow Integrations
NEPA21_Brain_EP_Results
NEPA21_comparison_with_Lipofectamine,_FuGENE_HD_and_NEON
NEPA21_EP_iPS_Cells_Results
NEPA21_Ex_Vivo-Results
NEPA21_In-Vivo_EP-Cerebellum-Pups_between_3-8_days_old
NEPA21_IN_Ovo_Results
NEPA21_In_Utero_Embryo_Publications
NEPA21_In_Vivo_RESULTS
NEPA21_In_Vivo_Tumor_EP
NEPA21_Retina_EP
NEPA21_Tobacco_Protoplast_Results
NEPA21_v_competing_Devices
NEPA21_v_CUY21EDIT_CUY21SC_Amaxa_BTXECM830
New Born Mouse and Rat (In Vivo) – Brain Ventricle (P0-P3)
New Born Mouse and Rat (In Vivo) – Brain Ventricle (P4-P8)
New Born Mouse and Rat (In Vivo) – Cornea
New Born Mouse and Rat (In Vivo) – Retina (P0-P3)
New Electroporator
New Products
Newsletter_2013-05
Novel 2-Step Pulse Regime Electroporation System for In Vivo and In Vitro Applicaitons – including cells in adherence and cells in suspension
Novel Sonoporation Platform for Gene Transfection, Drug Targeting and Sonodynamic Therapy for Cancer
NP60R-UBM Ultrasonic Biomicroscope for small animals
NSSB Mechanical Vitrabtion Instruments
Opticell
Organoid Electroporation Email
Organoid Request Trial
Oviduct EP with the NEPA21 – GONAD Technique
Plant (In Vivo) – Seed Embryo
Plant Protoplast Fusion By Electro Cell Fusion
Plant Protoplast Fusion by Electro-Cell-Fusion and by Electroporation
Plant Protoplast Fusion by Electroporation
Porcine Cloning – LF101/LF201
Postnatal_Cerebellum_EP_with_the_CUY699P7x6
Programmable Portable Freezer – no consumables
Protocol for in vivo electroporation into mouse and rat retina
Publications
Rabbit Cloning – LF101/LF201
Rat Heart – Sonoporation
Rat knockout
Rat-Knockout-Zygote_and_Oocyte_Electroporation
Request a Demonstration
ShortsinSum27
Silkworm – Egg
Simple Contact form
Single-Cell/Micro-Particle_Transfer-Glass_Micropipettes_and_Other_Accessories
Single-Cell/Micro-Particle_Transfer-Micro_Pick_and_Place_(MPP)_System_Configurations
Single_Cell_or_Small_Number_of_Cells_EP
Sonidel Sonoporation Data Results
Combined acupuncture and sonoporation for intradermal gene delivery
SONIDEL SP100 Sonoporator
SONIDEL STK10 Ultrasound Transfection Kit
Sonoporation
Chick Embryo Sonoporation
Page No Longer Exists
Video of KTAC4000 and KPS20 Probe in water
SonoPore KTAC-4000
SonoPore KTAC-4000 Probe Calibration Procedure
TEMPLATE ORGANOID WORK FLOWS
Test Accordian
test landing page
Test landing page
Test Page
Sub Test Page
Test Page Jump
Test Phone Call
Test remove title
Test spacing
Test spacing 2
Test1
Tissue Fragment (Ex Vivo) – Brain Slice
Tissue Fragment (Ex Vivo) – Skin Slice
Tissue Fragment (Ex Vivo) – Tissue Slice
TiYo LED Autofluorescence Quenching
Transformed Rice Plant Cultivation
Trial and Demo Information
Ultrasound Gene Transfer: One System for Non-invasive in vitro and in vivo applications
Ultrasound Targeted Gene Delivery
Vacuum & Electrical Pulse Method for Plant Seed Electroporation
Video of NEPA21 Applications
Xenopus Embryo (In Vivo) – Stage 12.5
Xenopus Tadpole – Tail
Zebrafish Brain Electrode Recommendation
Zebrafish Fin Electrode Recommendation
Zebrafish Retina Electrode Recommendation
Zebrafish_Electroporation
Sonoporation Consultancy and OEM
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April 2018
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