Mouse and Rat Small Tissue Electrode Recommendation and Protocol

Mouse and Rat Small Tissue Electrode Recommendations and Protocols:

Small Tissue:
Electrode Recommendations:
–           CUY700 series and
–           CUY701 series
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe that the electroporation methods described in:
–  Electroporation-mediated gene transfer system applied to cultured CNS neurons
–  Early subdivisions in the neural plate define distinct comptence for inductive signals
are appropriate, that is, placing an electrode on the top of a tissue which is on another electrode.  A number of electrode option recommendations are appropriate:

Electrode Option 1:

–           CUY700P2E and CUY700P1L or CUY195P0.5

Electrode Option 2:

–          CUY700P2E and CUY614 with CUY615, one CUY613P1 or CUY611P3-1

Cultured Mouse Embryo and Large Tissue:

Please refer to the following link: Cultured Mouse Embryo and Large Tissue EP

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See also Mouse and Rat Tissue/Brain Slice Recommendations