Mouse Embryo – Eye
Early Postnatal Mouse Eye and In Utero Embryo EP
Early Postnatal Mouse Eye EP
For your information:
With respect to gene delivery into Mouse Embryo Eye (and Brain) one can use the tungsten needle type electrode. However, as the mouse embryo is very small it is often hard to inject DNA into the embryo’s eye. Some clients have reported using the CUY675P5 electrode It is structurally possible to use this electrode for both Embryo (in utero) and Mouse Eye electroporation. (Please note the table below).
As one can see, however, there is a cut-out on one side of the CUY675P type electrodes. So, for this application we are not convinced that it is the optimum electrode for the application. Rather, our recommendation is for the CUY650P7 electrode. Note the attached CUY650P7 Technical Drawing.
This is the electrode most of our clients choose. It has a fully rounded set of electrode paddles and therefore covers a larger area of the target. In addition, this electrode has a much wider application range and can be used, for example, for or (whole) Cultured Mouse Embryos and Large Tissue EP. Dr. Matsuda uses the CUY650P7 electrode in his articles contained in the ‘EP with Retina’ below). Takahiko Matsuda and Constance L. Cepko Electroporation and RNA interference in the rodent retina in vivo and in vitro PNAS, Volume 101, Number 1, Pages 16-22, 6 January 2004 |
In Utero Embryo EP
The standard electrodes for In Utero Embryo EP are CUY650P3 and CUY650P5, which have circle electrodes without a cut. |
Mouse Embryo – Cornea
For gene delivery into Mouse Embryo – Cornea we recommend:
– CUY671P1 or Technical Drawing: CUY671P1 – CUY670 Technical Drawing: CUY670 Protocol Recommendation: – ‘Electroporation and RNA interference in the rodent retina in vivo and in vitro‘ – ‘Protocol for in vivo electroporation into mouse/rat retina’ – ‘Compensation by tumor suppressor genes during retinal development in mice and humans‘ For your further information, note the article titled: – ‘A role for ligand-gated ion channels in rod photoreceptor development’. In this article and ‘Electroporation and RNA interference in the rodent retina in vivo and in vitro’ above genes are transfected into mouse and rat retina. We believe that the method outlined in these articles can be applied to Gene Therapy |
Retina Cell EP – Results
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(See the cell images by clicking the cell names)
V: Viability, TE: Transfection Efficiency
A larger range of successfully electroporated cells is available at this link to our website: SONIDEL Cell Transfection Database