NEPA Porator for E Coli
Ohm Checkable Exponential Decay Wave Electroporator
Transformation / Transfection Applications
- E. coli
- Bacterial cells
- Yeasts
- Fungi
- Mammalian cells
Ohm check for controlling electroporation
Electroporation is generated by delivering an electric pulse(s), and the delivery of the electric pulse(s) at the same voltage and the same current with the same resistance under the ohm’s law is essential for achieving highly reproducible results.
The NEPA Porator can measure the electric impedance (that is almost equal to resistance) of the sample between the electrodes. Checking the impedance prior to electroporation is greatly helpful for avoiding inconstant electroporation, reviewing electroporation results and performing expected electroporation that will bring desired transformation results.
| Voltage setting; 500 V Checked sample impedance: 36 ohms |
Voltage setting; 500 V Checked sample impedance: 3,909 ohms |
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| Output electric pulse measured by oscilloscope |
Output electric pulse measured by oscilloscope |
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Specifications
Settings of exponential decay wave pulse
| Voltage | 100–500 V (5 V resolutionn) 500–3,000 V (100 V resolution) |
| Pulse length | None setting or 1.0-5.0 msec (0.5 msec resolution) |
| Number of pulses | 1 or 2 |
| NEPA Porator exponential decay wave pulse *Pulse length setting: none |
NEPA Porator exponential decay wave pulse *Pulse length setting: 1.0-5.0 msec |
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Measurements of output pulses
| Voltage | 100–3,000 V (1 V resolution) |
| Time constant | None setting: Measured value is displayed 1.0-5.0 msec setting: Set pulse length value is displayed |
| Number of pulses | 1 or 2 |
Others
| Impedance Measurement | 15–9,999 Ω (1 Ω resolution) 10,000–50,000 Ω (10 Ω resolution) |
| Save | 99 programs |
| Dimensions | 335 (W) x 205 (D) x 85 (H) mm |
| Weight | 3.2 kg |
*All features and specifications subject to change without notice.