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NEPA21 Electroporator
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Featured Device

Product Code: SONIDEL MB101

Desc:Microbubble for Ultrasound-mediated Transfection
Applic:Sonoporation Aid

Featured Electrode

Product Code: CUY900-13-5-2: Adherent-cell electrode

Electrodes for Electroporation
In Vitro EP: Adherent Cells: CUY900-13-5-2: Adherent cell electrodes, 13mmφ, 5 plates, 2mm gap (for 24-well plates/4-well dishes)

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Algae Transformation without cell-wall removal

Chlamydomonas Reinhardtii

Diatom Phaeodactylum Tricornutum

26-fold higher transfection efficiency than Bio-Rad 

Two recent publications citing the NEPA21 Super Electroporator:

–     Rapid transformation of Chlamydomonas Reinhardtii without cell-wall removal

–     Highly Efficient Transformation of the Diatom Phaeodactylum Tricornutum by Multi-Pulse Electroporation

Phaeodactylum tricornutum_1
Pictures: Phaeodactylum tricornutum
Provided courtesy of Dr. Kentaro Ifuku, Graduate School of Biostudies, Kyoto University and Dr. Jun-ichi Hotta, Graduate School of Science and Engineering, Yamagata University


Publication Information:
–  Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal
–  Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii
–  Isolation and characterization of mutants defective in the localization of LCIB, an essential factor for the carbonconcentrating mechanism in Chlamydomonas reinhardtii

–  A stable and efficient nuclear transformation system for the diatom Chaetoceros gracili
–  Highly Efficient Transformation of the Diatom Phaeodactylum tricornutum by Multi-Pulse Electroporation
–  Biochemical and physiological analyses of NADPH-dependent thioredoxin reductase isozymes in Euglena gracilis
–  Characterization and physiological role of two types of chloroplastic fructose-1,6-bisphosphatases in Euglena gracilis
–  Identification and characterization of cytosolic fructose-1,6-bisphosphatase in Euglena gracilis
–  Identification and functional analysis of peroxiredoxin isoforms in Euglena gracilis


Related links

Plant Protoplast Fusion by Electro-Cell-Fusion and by Electroporation
Plant Seed Electroporation (For Seeds of Rice Plant, Wheat and Barley, Silk Worm, Yeast, etc)
Algae Transformation without cell-wall removal
ECFG21 device for Hybridoma Production/Nuclear Transfer
ECFG Illustrated Applications

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