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Product Code: SONIDEL STK10
| Desc: | Ultrasound Transfection Positive Control |
Applic: | Sonoporation Transfection Kit |
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Product Code: CUY560-10-0.5
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Application: Electrodes for Electroporation |
Description: Stainless Steel, Parallel fixed needle electrodes, 10mm tip length, 10mm gap, 0.5mmφ |
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NEPA21 Electroporator
Super Electroporator NEPA21
The NEPA21 Electroporator is our latest and most sophisticated electroporation device to-date. It is the successor to our hugely successful CUY21EDIT and CUY21SC electroporator range.
The NEPA21 Electroporator is based on the same platform and core technology that made the CUY21EDIT and CUY21SC devices leaders in their class. The NEPA21 can do in one device what each of the EDIT and SC did individually, i.e., it combines BOTH the wider voltage range of the EDIT and the enhanced sensitivity at lower voltages of the SC.
The NEPA21 has been bench-marked and tested against the CUY21 series to ensure that it delivers the exact same protocol results as the EDIT and SC devices for each and every published application. In addition, the NEPA21 also offers other novel attributes that extend its application range even further:
– a 2-Step Pulse: A Electroporation (Poring) Pulse (a high voltage & short duration pulse) and a Transferring (Driving) Pulse (a low voltage with multiple duration settings pulses)
– the NEPA21 not only incorporates the full In Vivo, In Utero, In Ovo and Ex Vivo application ranges of the CUY21EDIT and CUY21SC devices, but it also includes a completely new and novel In Vitro application range
– it can be targeted at BOTH In Vitro (Suspended Cells and Adherent Cell) and In Vivo applications
– lower operating-cost advantages for both Suspended Cells and Adherent Cells (in commercially available cell culture plates) and In Vivo applications (there is no requirement for expensive special reagents)
– delivers High Transfection Efficiency and Viability
– a voltage output-range that matches the published extended-output voltage protocols of the CUY21EDIT device
– an enhanced voltages output-control that matches the renowned sensitivity of the CUY21SC device at low voltage settings
NEPA21 Square Wave Electroporator
In Vitro & In Vivo Transfection
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High Transfection Efficiency & Viability WITHOUT Special Buffers
.
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Features:
For PRIMARY CELL, STEM CELLS and ANY CELL Line:
– With Electroporation Cuvettes For Cells in Suspension
– With Adherent Cell Electrodes For Cells in Adherence
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For MOUSE/RAT BRAIN, EYE, MUSCLE, SKIN, LIVER, KIDNEY, TESTIS, ETC
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For MOUSE/RAT EMBRYO:
– CEREBRAL, CORTEX, HIPPOCAMPUS, SPINAL CORD, ETC
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For CHICK EMBRYO:
– NEURAL TUBE, LIMB BUD, DIGESTIVE ORGAN, ETC
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For TISSUE SLICE, BRAIN SLICE, ORGAN, WHOLE EMBRYO, ETC
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.
The NEPA21 Electroporator covers the full application range of its predecessor CUY21EDIT and CUY21SC electroporators
The NEPA21 Electroporator complies with the CE Certification, UL standard and CSA standard.
Request a Demo
And see how the NEPA21 performs with your Cell Lines
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Novel 3-Step Multiple Electroporation Pulse:
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The 3-Step pulse regime with voltage decay and polarity exchange facilitates more highly controlled electroporation pulse delivery.This results in higher transfection efficiency and higher cell viability WITHOUT a requirement for specialised buffers.. |
Step 1 |
Membrane ‘Poration’ Pulse Mode:High Voltage, Short Duration, Single/Multiple Pulse(s), Voltage DecayThis poration pulse forms pores in the cell membrane with minimum residual cell damage . |
Step 2 |
Transfer Pulse Mode:Low Voltage, Long Duration, Multiple Pulses, Voltage Decay.This transfer pulse is for electrokinetic delivery of the target molecules (DNA, RNA, etc.) into cells with minimum residual cell damage . |
Step 3 |
Polarity Exchanged Transfer PulseThis can improve the transfection efficiency and again, minimise residual cell damage. |
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... |
Applications with Electroporation Cuvettes
The NEPA21 Electroporator makes it possible to achieve high transfection efficiency and viability without recourse to special buffers for PRIMARY CELLS, STEM CELLS and difficult-to-transfect cells asIMMUNE CELLS, BLOOD CELLS, ETC.
1) Transfection into Primary Cells
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– BMMC Primary Mouse Bone Marrow-Derived Mast cells
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Viability: 64% |
Transfection Efficiency: 78% |
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FACS Data
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..Our transfection efficiency data is a ratio of transfected cells to survived cells.(E.g. If the original cells are 10, viable cells are 8 and transfected cells are 6, the viability is 80% (8/10) and the transfection efficiency is 75% (6/8).) |
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– Primary Mouse Neurons, Cerebral Cortex
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Viability: 80% |
Transfection Efficiency: 70% |
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– Primary Rat Bulbar Nerve Cells
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Viability: 80% |
Transfection Efficiency: 75% |
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– HASM Primary Human Airway Smooth Muscle cells
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 |
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Viability: 90% |
Transfection Efficiency: 80% |
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– Primary Human Endometrial Stromal Cells
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Viability: 95%. |
Transfection Efficiency: 90%. |
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Disposable Kit Comparison
NEPA21 (Nepa Gene) |
Device: N (Company L) |
Device: N (Company I) |
Cuvettes ONLYNo Special Buffers |
Transfection KitsWith Special Buffers |
Transfection KitsWith Special Buffers |
Cost per One SampleEuro 1.80 (€90.00/50 pcs) |
Cost per One SampleEuro 18.60 (€186.05/10 RCT) |
Cost per One SampleEuro 15.00 (€375.00/25 Tips) |
*Euro Price. |
.Is your laboratory still using a transfection device that requires expensive disposable kits?Many of our clients cite the NEPA21’s lower running cost as a major factor in deciding to purchase the device.The running cost of NEPA21 is much lower than other transfection devices. . |
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2) Transfection into Cell Lines
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Please click the following links for the corresponding Cell Image |
NEPA21 Cell Transfection Data Listing
V: Viability
TE: Transfection Efficiency
Cell Lines: With Electroporation Cuvettes
Cell Line |
Description, Species |
Characteristics, etc.
|
Viability |
Efficiency |
Photo |
HeLa |
Human Cervical Carcinoma cells
|
Epithelial, Immortalized
Adherent
|
95%
|
95%
|
Yes |
HeLa-K |
Human Cervical Carcinoma cells |
Epithelial, Immortalized
Adherent |
90% |
90% |
|
293
(HEK293) |
Human Embryonic Kidney cells
|
Epithelial , Adherent
Adenovirus type 5
|
90%
|
90%
|
|
293
(HEK293) |
Human Embryonic Kidney cells |
Epithelial , Adherent |
90% |
70% |
Yes |
293T
(HEK293T) |
Human Embryonic Kidney cells
|
Epithelial , Adherent
SV40 large T antigen
|
90%
|
95%
|
Yes |
TIG-3 |
Human Embryonic Lung Fibroblasts
|
Diploid, Normal
Adherent |
90% |
80% |
Yes |
TIG-7 |
Human Embryonic Lung Fibroblasts
|
Diploid, Normal
Adherent
|
89%
(FACS)
|
76%
(FACS)
|
Yes |
MRC-5 |
Human Embryonic Lung Fibroblasts |
Diploid, Normal
Adherent |
85% |
90% |
Yes |
SUSM-1 |
Human Fibroblasts |
Fetal Liver, Epithelial, Normal, Immortalized |
77%
(FACS) |
71%
(FACS) |
Yes |
KMST-6 |
Human Fibroblasts |
Whole Embryo, Epithelial
Normal, Immortalized |
70% |
60% |
Yes |
hTERT-BJ1 |
Human Fibroblast |
Normal, Immortalized, Adherent |
Good |
31%
(FACS) |
|
HUVEC |
Human Umbilical Vein Endothelial cells |
Normal, Adherent |
70% |
75% |
|
HT1080 |
Human Fibrosarcoma cells |
Connective Tissue
Acetabulum, Adherent |
93% |
81% |
Yes |
MIA-PaCa-2 |
Human Pancreatic Carcinoma cells |
Epithelial , Adherent |
80% |
77% |
Yes |
HepG2 |
Human Hepatoma cells |
Epithelial , Adherent |
80% |
76% |
Yes |
HuH-7 |
Human Hepatoma cells |
Epithelial , Adherent |
70% |
60% |
Yes |
H69 |
Human Small-Cell Lung Cancer cells |
Adherent |
90% |
85% |
Yes |
H1299
(NCI-H1299) |
Human Lung Cancer cells |
Non-Small, Epithelial
Adherent |
90% |
90% |
|
HSC-2 |
Human Squamous Carcinoma cells |
Oral , Adherent |
90-95% |
98% |
Yes |
HSC-3 |
Human Squamous Carcinoma cells |
Tongue , Adherent |
90-95% |
98% |
Yes |
HSC-4 |
Human Squamous Carcinoma cells |
Tongue, Adherent |
80% |
34% |
Yes |
Ca9-22 |
Human Squamous Carcinoma cells |
Gingival, Adherent |
60% |
60% |
Yes |
HGF |
Human Gingival Fibroblasts |
Normal, Adherent |
Excellent |
Excellent |
Yes |
MCF-7 |
Human Breast Cancer cells |
Epithelial, Adherent |
90% |
90-95% |
|
T47D |
Human Breast Cancer cells |
Ductal Carcinoma
Epithelial, Adherent |
90% |
80-90% |
|
BT-20 |
Human Breast Cancer cells |
Adherent |
70% |
80% |
Yes |
MCF-10A |
Human Brest cells |
Normal, Mammary Epithelial
Adherent |
Excellent |
Excellent |
Yes |
NUGC-3 |
Human Gastric Carcinoma cells |
Adherent |
73%
(FACS) |
68%
(FACS) |
|
A549 |
Human Lung Adenocarcinoma cells |
Epithelial, Adherent |
80-90% |
90% |
|
TE-1 |
Human Esophageal Carcinoma cells |
Adherent |
80-90% |
41% |
Yes |
TE-8 |
Human Esophageal Carcinoma cells |
Adherent |
80-90% |
40% |
Yes |
LNCaP |
Human Prostate Carcinoma |
Epithelial, Adherent |
71%
(FACS) |
90%
(FACS) |
|
PC-3 |
Human Prostate Cancer cells |
Epithelial, Adherent |
90% |
95% |
|
RKO |
Human Colorectal Carcinoma cells |
Adherent |
80% |
40% |
|
SKOV-3 |
Human Ovarian Carcinoma cells |
Adherent |
90% |
90% |
Yes |
OVCAR-3 |
Human Ovarian Carcinoma cells |
Adherent |
90% |
79% |
Yes |
RMG-1 |
Human Ovarian Clear Cell
Adenocarcinoma |
Adherent |
97% |
67% |
Yes |
CCS |
Clear Cell Sarcoma |
Adherent |
47% |
55% |
|
SK-N-SH |
Human Neuroblastoma cells |
Epithelial, Adherent |
95% |
95% |
Yes |
SH-SY5Y |
Human Neuroblastoma cells |
Adherent |
60% |
90% |
Yes |
KG-1-C |
Human Oligodendroglial cells |
Adherent |
85% |
60% |
Yes |
A172 |
Human Glioblastoma cells |
Adherent |
80-90% |
70% |
Yes |
1321N1 |
Human Astrocytoma Cells |
Adherent |
80% |
80% |
Yes |
HaCaT |
Human Keratinocyte cells |
Normal, Immortalized
Adherent |
40% |
80% |
Yes |
CCD1079sk |
Human Skin Fibroblasts |
Adherent |
40% |
53%
(FACS) |
Yes |
iHAM-4 |
Human Amniotic Mesenchymal cells |
Adherent |
59% |
95% |
|
iHAE-7 |
Human Amniotic Epithelial cells |
Adherent |
70% |
40% |
|
HTR-8/Svneo |
Human Trophoblast cells |
Adherent |
95% |
67% |
Yes |
RPE |
Retinal Pigment Epithelium cells |
Adherent |
90% |
70% |
|
RPTEC |
Renal Proximal Tubule Epithelial cells |
Adherent |
70% |
85% |
|
UT-7/TPO |
Human Megakaryocytic cells |
Suspension |
51% |
46% |
Yes |
Jurkat |
Human T-cell Leukemia cells |
Round Single, Blood, Suspension |
90% |
85% |
Yes |
Nalm-6 |
Human B-cell Precursor Leukemia cells |
Blood, Suspension |
67%
(FACS) |
70%
(FACS) |
|
MY |
Human Acute Lymphocytic Leukemia cells |
Myelomonocytic, Suspension |
94%
(FACS) |
22%
(FACS) |
|
PALL-2 |
Human Acute Leukemia cells |
Acute Lymphoblastic |
60% |
27%
(FACS) |
|
KG-1 |
Human Acute Myeloid Leukemia cells |
Susension |
50% |
50% |
Yes |
USCD/AML1 |
Human Leukema cells |
Suspension |
50% |
50% |
|
Namalwa |
Human Burkitt’s Lymphoma cells |
B lymphocyte, Blood
Suspension |
70% |
70-80% |
|
Raji |
Human Burkitt’s Lymphoma cells |
B lymphocyte, Blood
Suspension |
74%
(FACS) |
53%
(FACS) |
|
Mutu I |
Human Burkitt Lymphoma cells |
EBV Positive, Suspension |
87%
(FACS) |
91%
(FACS) |
Yes |
Mutu III |
Human Burkitt Lymphoma cells |
EBV Positive, Suspension |
54%
(FACS) |
92%
(FACS) |
|
HL-60 |
Human Promyelocytic Leukemia cells |
Suspension |
80% |
80% |
|
LCL |
Human Burkitt’s Lymphoma cells |
Immortalized, Suspension |
55%
(FACS) |
43%
(FACS) |
Yes |
Ramos-BlueTM |
Human Basophilic Leukemia cells |
Suspension |
80% |
55% |
Yes |
KU812 |
Human Basophilic Leukemia cells |
Suspension |
96%
(FACS) |
42%
(FACS) |
|
K562 |
Human Chronic Myelogenous Leukemia cells |
Suspension |
91%
(FACS) |
99%
(FACS) |
Yes |
THP-1 |
Human Acute Moncytic Leukemia cells |
Suspension |
76% |
63% |
Yes |
|
|
|
|
|
|
NIH/3T3 |
Mouse Embryonic Fibroblasts |
Swiss NIH , Embryo
Adherent |
74%
(FACS) |
82%
(FACS) |
Yes |
3T3-L1 |
Mouse Preadipocyte |
Fibroblast like |
90% |
90% |
Yes |
MEF |
Mouse Embryonic Fibroblasts |
Adherent |
80% |
90% |
Yes |
MEF |
Mouse Embryonic Fibroblasts |
Adherent |
75% |
70% |
Yes |
P19C6 |
Mouse Embryonic Carcinoma Cells |
Adherent |
90% |
50% |
Yes |
L |
Mouse Fibroblasts |
Adherent |
90% |
60-70% |
Yes |
MC3T3-E1 |
Mouse Osteoblastic cells |
Fibroblastic, Adherent |
85% |
75% |
Yes |
LLc1
(LL/2) |
Lewis Lung Cell Carcinoma 1 cells |
Adherent |
87%
(FACS) |
81%
(FACS) |
Yes |
C2C12 |
Mouse Myoblast cells |
Adherent |
80% |
70% |
Yes |
4T1 |
Mouse Breast Cancer cells |
Adherent |
90% |
95% |
Yes |
Colon-26 |
Mouse Colon Adenocarcinoma cells |
Adherent |
95% |
90% |
Yes |
MS-1 |
Mouse Pancreatic Endothelial cells |
Adherent |
90% |
90% |
|
|
ddy Mouse Endometrial cells |
Fibroblast, Adherent |
60% |
80% |
|
Neuro-2a |
Mouse Neuroblastoma Cells |
Adherent |
90% |
90% |
Yes |
BV-2 |
Mouse Microglial cells |
Adherent |
92% |
60% |
Yes |
WR19L |
Mouse T-cell Lymphoma cells |
Suspension |
92%
(FACS) |
60%
(FACS) |
Yes |
BA/F3 |
Mouse pro-B cells |
Suspension |
90% |
90% |
|
RAW264.7 |
Mouse Macrophage-like cells |
Monocyte-like, Adherent |
70% |
56% |
Yes |
MIN6 |
Mouse Pancreatic Beta cells |
Suspension |
57%
(FACS) |
71%
(FACS) |
Yes |
|
Mouse Pancreatic Islet Beta cells |
|
60% |
40% |
Yes |
MEL |
Mouse Erythroleukemia cells |
Suspension |
70%
(FACS) |
50%
(FACS) |
|
XS106 |
Mouse Dendritic Cell |
Suspension |
61% |
45% |
Yes |
mDC |
Mouse Myeloid Dendritic cells |
Suspension |
79%
(FACS) |
72%
(FACS) |
|
MC/9 |
Mouse Mast cells |
Suspension |
76%
(FACS) |
84%
(FACS) |
Yes |
BMBa |
Mouse Bone Marrow-Derived Basophils |
Suspension |
45%
(FACS) |
67%
(FACS) |
Yes |
ATDC5 |
Mouse pre-Chondrocytic cells |
|
62% |
14% |
Yes |
|
|
|
|
|
|
PC12 |
Rat Adrenal Pheochromocytoma cells |
Adherent |
90% |
70% |
Yes |
H9c2 |
Rat Ventricular Myoblasts |
Skeletal Muscle-like
Adherent |
71%
(FACS) |
82%
(FACS) |
Yes |
REF |
Rat Embryonic Fibroblasts |
Adherent |
90% |
99% |
Yes |
RDF |
Rat Dermal Fibroblasts |
Adherent |
90% |
40% |
|
C6 |
Rat Glioma cells |
Fibroblast-like, GFAP Positive, Adherent |
80% |
67% |
Yes |
RSC96 |
Rat Schwann cells |
Adherent |
70% |
85% |
Yes |
TtT/GF |
Rat Anterior Pituitary cells |
Folliculostellate, Adherent |
65% |
83% |
Yes |
UMR106 |
Rat Osteoblastic cells |
Adherent |
80% |
70% |
|
|
|
|
|
|
|
CHO |
Chinese Hamster Ovary cells |
Epithelial, Adherent |
74% |
90% |
Yes |
CHO-K1 |
Chinese Hamster Ovary cells |
Epithelial, Adherent |
95% |
95% |
Yes |
CHO-S |
Chinese Hamster Ovary cells |
Epithelial, Suspension |
Excellent |
56%
(FACS) |
|
DT40 |
Chicken B cells |
Suspension |
38% |
40%
(FACS) |
Yes |
MDCK |
Madrin-Darby Canine Kidney cells |
Epithelial-like, Adherent |
90% |
95% |
Yes |
BFF |
Bovine Fetal Fibroblasts |
Adherent |
78%
(FACS) |
72%
(FACS) |
Yes |
A6 |
Xenopus Kidney Epithelial cells |
Adherent |
90% |
60% |
Yes |
GTS9 |
Ginbuna Thymus cells |
Adherent |
50% |
30% |
Yes |
Vero |
African Green Monkey Kidney cells |
Epithelial-like, Adherent |
58% |
35% |
|
SEC |
Bovine T cells |
Suspension |
80% |
21%
(FACS) |
|
.
Stem Cells, EC Cells and iPS Cells: With Electroporation Cuvettes
Cell Line |
Description, Species |
Characteristics, etc. |
Viability |
Efficiency |
Photo |
|
Human Mesenchymal stem cells |
Primary, Adherent
Cord blood derived |
78%
(FACS) |
75%
(FACS) |
Yes |
TS |
Mouse Trophoblast Stem cells |
Adherent |
59% |
47% |
Yes |
TT2 |
Mouse TT2 ES cells |
Adherent |
50-60% |
50-60% |
Yes |
|
Mouse ES cells |
Adherent |
80% |
75% |
Yes |
|
Rat WDA ES-like cells |
Adherent |
60% |
80% |
|
|
Mouse iPS Cells |
Origin: Mouse Fibroblast, Adherent |
89% |
47% |
Yes |
|
Mouse Neurospheres
Neural Stem Derrived from Mouse SVZ |
|
Excellent |
Excellent |
Yes |
|
Mouse Neural Stem cells |
Primary Adherent |
Good |
37% |
Yes |
.
Primary Cells: With Electroporation Cuvettes
Cell Line |
Description, Species |
Characteristics, etc. |
Viability |
Efficiency |
Photo |
HASM |
Human Airway Smooth Muscle cells |
Primary, Adherent |
90% |
80% |
Yes |
|
Human Endometrial Stromal cells |
Primary Adherent |
95% |
90% |
Yes |
|
Human Uterine Cervical Fibroblast |
Primary, Adherent |
65% |
90% |
Yes |
|
Human Amniotic Mesenchymal cells |
Primary, Adherent |
70% |
40% |
|
|
Human T cells |
Primary, Suspension |
58%
(FACS) |
90%
(FACS) |
Yes |
|
Mouse Cerebral Cortex Neurons
(Embryonic day14) |
Primary, Adherent |
80% |
70% |
Yes |
|
Mouse Hippocampal Neurons
(Embryonic day 14) |
Primary, Adherent |
80% |
60% |
|
|
Mouse Basal Ganglia Primordium
(Embryonic day 13.5) |
Primary, Adherent |
91%
(FACS) |
71%
(FACS) |
|
|
Mouse Microglia cells |
Primary, Adherent |
60-70% |
23% |
Yes |
MEF |
Mouse Embryonic Fibroblasts |
Primary, Adherent |
90% |
85% |
Yes |
|
Mouse Embryonic Skin Fibroblasts |
Primary, Adherent |
90% |
50% |
Yes |
BMMC
(BMMCs) |
Mouse Bone Marrow-Derived Mast cells |
Primary, Suspension |
64%
(FACS) |
78%
(FACS) |
Yes |
|
Mouse Primary Liver cells |
Primary, Adherent |
70% |
50% |
Yes |
|
Mouse Stromal cells |
Primary, Adherent |
90% |
30% |
|
|
Rat Cerebral Cortex Neurons
(Embryonic day 16) |
Primary, Adherent |
64% |
78% |
Yes |
|
Rat Hippocampal Neurons |
Primary, Adherent |
60% |
80% |
|
|
Rat Bulbar Neurons
(Embryonic day 15) |
Primary, Adherent |
80% |
75% |
Yes |
|
Rat Cerebellar Granule cells |
Primary, Adherent |
70% |
80% |
|
|
Rat Meningeal Fibroblasts
(Postnatal day 3) |
Primary, Adherent |
90% |
95% |
Yes |
OEC |
Rat Olfactory Ensheathing cells
(Postnatal week 3) |
Primary, Adherent |
93% |
46% |
Yes |
|
Chick Embryonic Fibroblasts |
Primary Adherent |
80% |
90% |
Yes |
|
Chick Embryonic Cerebellar Granule cells
(Embryonic day 11) |
Primary, Adherent |
86% |
83% |
Yes |
.
Cells Lines in Adherence: With Adherent-Cell Electrodes
Cell Line |
Description, Species |
Characteristics, etc. |
Viability |
Efficiency |
Photo |
SH-SY5Y |
Human Neuroblastoma cells |
Adherent |
90% |
50% |
Yes |
EPC |
Human Endothelial Progenitor cells |
Adherent |
|
|
Yes |
3T3-L1 |
Mouse Preadipocytes |
Adherent |
90% |
70% |
Yes |
C6 |
Rat Glioma cells |
Fibroblast-like, GFAP Positive, Adherent |
80% |
39% |
Yes |
.
Primary Cell in Adherence: With Adherent-Cell Electrodes
Cell |
Characteristics |
Viability |
Efficiency |
Photo |
Human Epidermal Keratinocyte cell |
Primary, Adherent |
90% |
30% |
|
Mouse Cerebral Cortex Neurons
(Embryonic day 15) (6 DIV) |
Primary, Adherent |
Excellent |
Excelent |
Yes |
Mouse Hippocampal Neurons
(Embryonic day 14) (4 DIV) |
Primary, Adherent |
60% |
50% |
|
Mouse Hippocampal Neurons
(Embryonic day 18) (2 DIV) |
Primary, Adherent |
85% |
54% |
Yes |
Mouse Stromal cells
(1-month cultured) |
Primary, Adherent |
90% |
50% |
|
Mouse Glial cells
(14 DIV) |
Primary, Adherent |
80% |
50% |
Yes |
Rat Granulosa cells |
Primary, Adherent |
Excellent |
41% |
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Rat Neurons
(P7 rat hippocampus. After 11 DIV) |
Primary, Adherent |
100% |
50% |
Yes |
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Plant Protoplasts
Cell |
Characteristics |
Luciferase Activity
(CPS) |
Tabacco Protoplasts |
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20,312 |
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Applications with Adherent Cell Electrodes
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By using the Adherent Cell Electrode CUY900 series, it is now possible to transfer DNA/RNA directly into CELLS IN ADHERENCE in commercially available 24-well plates. |
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● Gene Transfection into Primary Neurons (Adherent) by Electroporation
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pCAGGS-EGFP plasmid was transferred into primary neurons cultured for 6 days in adherent state.The neurons were prepared from E15 mouse embryo cerebral cortex. A): .2-step pulse electroporation using the electrodes for adherent cells (CUY900-13-3-5) B): .EGFP fluorescence image of the neurons 2 days after electroporation C): .High magnification image of Figure B …....Many robust EGFP signals suggest high transfection efficiency. D): High magnification image of Figure C (x40) …....Neurites are shown clearlyDepartment of Neurochemistry, National Institute of Neuroscience.. |
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Applications with In Vivo Electrodes
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. NEPA21 electroporator together with its specially designed CUY-series in vivo electrode range is optimised to facilitate the transfer of DNA/RNA into:.………………….MOUSE/RAT Muscle, Skin, Liver, Kidney, Testis, Ovary, Brain, Retina, Cornea, Etc.. |
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………….MUSCLE…………………………………….SKIN……………………..TESTIS |
…………………..RETINA…………………..BRAIN……………………EGG |
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Please click here for further DETAILED APPLICATION INFORMATION
Please click here for further ELECTRODE INFORMATION
Please click here for NEPA21/CUY21 Publications
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Applications withIn Utero/Ex Utero Electrodes
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.The NEPA21 electroporator together with its specially designed CUY-series In Utero/Ex Utero electrode range is optimised to facilitate the transfer of DNA/RNA into:.……………………MOUSE/RAT Embryo (Cerebral Cortex, Hippocampus, Spinal Cord, Etc.). |
● Transfection into Mouse/Rat Embryonic Brain
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Please click here for further DETAILED APPLICATION INFORMATION
Please click here for further ELECTRODE INFORMATION
Please click here for NEPA21/CUY21 Publications
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Applications with In Ovo Electrodes
● Transfection into Chick Embryos
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, hematopoietic system (B, F), notochord (C, G), and lateral plate mesoderm (D, H).jpg)
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Transfection into the central canal |
Transfection into the somites (A, E), hematopoietic system (B, F), notochord (C, G), and lateral plate mesoderm (D, H) |
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Please click here for further DETAILED APPLICATION INFORMATION
Please click here for further ELECTRODE INFORMATION
Please click here for NEPA21/CUY21 Publications
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Applications with Ex Vivo Electrodes
● Transfection into Brain Tissue
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Transfection into BRAIN SLICE
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Transfection into the embryonic tissue
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Please click here for further DETAILED APPLICATION INFORMATION
Please click here for further ELECTRODE INFORMATION
Please click here for NEPA21/CUY21 Publications
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● Transfection into Rat Embryos in the Whole Embryo Culture System
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Transfection into the developing rat cortex |
Transfection into the rat spinal cord |
.Please click here for further DETAILED APPLICATION INFORMATIONPlease click here for further ELECTRODE INFORMATION Please click here for NEPA21/CUY21 Publications |
NEPA21 (3-Step) Electroporator
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Electrodes and Accessories required to transfect
Adherent Cells
o CUY900-24-3: Adherent cell electrodes with 3 plates (for 24 well plates)
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o CUY900-24-5: Adherent cell electrodes with 5 plates (for 24 well plates)
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Electrodes and Accessories required to transfect
Cell Suspensions
o EP cuvettes, with pipette
o CU500 Cuvette Chamber
o CU600 Cuvette Stand Holder
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