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NEPA21 Electroporator
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Transgenic Mouse Zygote Electroporation: NEPA21
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2
Product Code: SONIDEL MB101

Desc:Microbubble for Ultrasound-mediated Transfection
Applic:Sonoporation Aid


Featured Electrode



Product Code: CUY557-2

Application:
Electrodes for Electroporation
Description:
Stainless Steel, Parallel fixed needle electrodes, 1mm tip length, 2mm gap, 0.2mmf

Information
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NEPA21 / CUY21 Publications by Research Interest

NEPA21 and CUY21 Publications List

APPLICATION

RAT & MOUSE

–  Embryo (In Utero)
–  Cultured Embryo
–  Brain
–  Brain (Newborn)
–  Brain Tissue
–  Retina (In Vitro & Newborn)
–  Liver
–  Kidney
–  Testis & Ovary
–  Muscle
–  Joint
–  Skin

CHICK

–  Embryo (In Ovo)
–  Embryo (New Culture)
–  Chick

ZEBRAFISH

–  Retina
–  Fin

XENOPUS

HONEY BEE

CRICKET

PLANARIAN

ADHERENT CELL

ES CELLS

ELECTROCHEMOTHERAPY

.

APPLICATION

PUBLICATION TITLE

ELECTRODE RECOMMENDATION

VOLTAGE

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No OF PULSES

RAT & MOUSE EMBRYO      (In Utero)

Prozorovski et al.

Sirt1 contributes critically to the redox-dependent fate of neural progenitors

Nature Cell Biology, Volume 10, Number 4, Pages 385-394, April 2008

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Muraguchi et al.

RECK modulates Notch signaling during cortical neurogenesis by regulating ADAM10 activity

Nature Neuroscience, Volume 10, Number 7, Pages 838-845, July 2007

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Akihide Takeuchi and Dennis D. M. O’Leary

Radial Migration of Superficial Layer Cortical Neurons Controlled by Novel Ig Cell Adhesion Molecule MDGA1

The Journal of Neuroscience, Volume 26, Issue 17, Pages 4460-4464, 26 April 2006

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Tetsuichiro Saito

In vivo electroporation in the embryonic mouse central nervous system

Nature Protocols, Volume 1, Number 3, Pages 1552-1558, 2006

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Saba et al.

Commissural neuron identity is specified by a homeodomain protein, Mbh1, that is directly downstream of Math1

Development, Volume 132, Issue 9, Pages 2147-2155, May 2005

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Eiko Nakahira and Shigeki Yuasa

Neuronal generation, migration, and differentiation in the mouse hippocampal primoridium as revealed by enhanced green fluorescent protein gene transfer by means of in utero electroporation

The Journal of Comparative Neurology, Volume 483, Issue 3, Pages 329-340, 14 March 2005

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Kamon Sanada and Li-Huei Tsai

G Protein betagamma Subunits and AGS3 Control Spindle Orientation and Asymmetric Cell Fate of Cerebral Cortical Progenitors

Cell, Volume 122, Issue 1, Pages 119-131, 15 July 2005

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Itsuki Ajioka and Kazunori Nakajima

Birth-date-dependent segregation of the mouse cerebral cortical neurons in reaggregation cultures

European Journal of Neuroscience, Volume 22, Issue 2, Pages 331-342, July 2005

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Takiguchi-Hayashi et al.

Generation of Reelin-Positive Marginal Zone Cells from the Caudomedial Wall of Telencephalic Vesicles

The Journal of Neuroscience, Volume 24, Issue 9, Pages 2286-2295, 3 March 2004

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Nguyen et al.

A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons

Nature Cell Biology, Volume 6, Number 7, Pages 595-608, July 2004

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Hatakeyama et al.

Hes genes regulate size, shape and histogenesis of the nervous system by control of the timing of neural stem cell differentiation

Development, Volume 131, Issue 22, Pages 5539-5550, November 2004

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Saba et al.

Mammalian BarH1 Confers Commissural Neuron Identity on Dorsal Cells in the Spinal Cord

The Journal of Neuroscience, Volume 23, Issue 6, Pages 1987-1991, 15 March 2003

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Kawauchi et al.

The in vivo roles of STEF/Tiam1, Rac1 and JNK in cortical neuronal migration

The EMBO Journal, Volume 22, Number 16, Pages 4190-4201, August 2003

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Gupta et al.

Layering defect in p35 deficiency is linked to improper neuronal-glial interaction in radial migration

Nature Neuroscience, Volume 6, Number 12, Pages 1284-1291, December 2003

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Hidenori Tabata and Kazunori Nakajima

Multipolar Migration: The Third Mode of Radial Neuronal Migration in the Developing Cerebral Cortex

The Journal of Neuroscience, Volume 23, Issue 31, Pages 9996-10001, 5 November 2003

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Xie et al.

Serine 732 Phosphorylation of FAK by Cdk5 Is Important for Microtubule Organization, Nuclear Movement, and Neuronal Migration

Cell, Volume 114, Issue 4, Pages 469-482, 22 August 2003

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Tomomi Fukuchi-Shimogori and Elizabeth A Grove

Emx2 patterns the neocortex by regulating FGF positional signaling

Nature Neuroscience, Volume 6, Number 8, Pages 825-831, August 2003

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Ohtsuka et al.

Roles of the Basic Helix-Loop-Helix Genes Hes1 and Hes5 in Expansion of Neural Stem Cells of the Developing Brain

The Journal of Biological Chemistry, Volume 276, Issue 32, Pages 30467-30474, 10 August 2001

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H. Tabata and K. Nakajima

Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex

Neuroscience, Volume 103, Issue 4, Pages 865-872, 4 April 2001

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RAT & MOUSE CULTURED EMBRYO

Shibuya et al.

Isolation and Characterization of Vasohibin-2 as a Homologue of VEGF-Inducible Endothelium-Derived Angiogenesis Inhibitor Vasohibin

Arteriosclerosis, Thrombosis, and Vascular Biology, Volume 26, Issue 5, Pages 1051-1057, May 2006

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Tadashi Nomura and Noriko Osumi

Misrouting of mitral cell progenitors in the Pax6/small eyerat telencephalon

Development, Volume 131, Issue 4, Pages 787-796, February 2004

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Kawabata et al.

Electroporation-mediated gene transfer system applied to cultured CNS neurons

Neuroreport, Volume 15, Issue 6, Pages 971-975, 29 April 2004

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Masanori Takahashi and Noriko Osumi

Pax6 regulates specification of ventral neurone subtypes in the hindbrain by establishing progenitor domains

Development, Volume 129, Issue 6, Pages 1327-1338, March 2002

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Takahashi et al.

Manipulating gene expressions by electroporation in the developing brain of mammalian embryos

Differentiation, Volume 70, Issue 4-5, Pages 155-162, June 2002

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Noriko Osumi and Takayoshi Inoue

Gene Transfer into Cultured Mammalian Embryos by Electroporation

Methods, Volume 24, Issue 1, Pages 35-42, May 2001

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RAT  &  MOUSE BRAIN

Barnabe-Heider et al.

Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation

Nature Methods, Volume 5, Number 2, Pages 189-196, February 2008

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Inoue et al.

Locus-Specific Rescue of GluR1 NMDA Receptors in Mutant Mice Identifies the Brain Regions Important for Morphine Tolerance and Dependence

The Journal of Neuroscience, Volume 23, Issue 16, Pages 6529-6536, 23 July 2003

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Tanaka et al.

Up-regulation of Protein-disulfide Isomerase in Response to Hypoxia/Brain Ischemia and Its Protective Effect against Apoptotic Cell Death

The Journal of Biological Chemistry, Volume 275, Issue 14, Pages 10388-10393, 7 April 2000

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RAT & MOUSE (Newborn) BRAIN

Renaud et al.

Plexin-A2 and its ligand, Sema6A, control nucleus-centrosome coupling in migrating granule cells

Nature Neuroscience, Volume 11, Number 4, Pages 440-449, April 2008

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Boutin et al.

Efficient In Vivo Electroporation of the Postnatal Rodent Forebrain

PLoS ONE, Volume 3, Issue 4, e1883, 2 April 2008

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Umeshima et al.

Microtubule-based nuclear movement occursindependently of centrosome positioning in migrating neurons

PNAS, Volume 104, Number 41, Pages 16182-16187, October 9, 2007

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RAT  &  MOUSE BRAIN TISSUE

Yozu et al.

The Caudal Migratory Stream: A Novel Migratory Stream of Interneurons Derived from the Caudal Ganglionic Eminence in the Developing Mouse Forebrain

The Journal of Neuroscience, Volume 25, Issue 31, Pages 7268-7277, 3 August 2005

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Kawabata et al.

Electroporation-mediated gene transfer system applied to cultured CNS neurons

Neuroreport, Volume 15, Issue 6, Pages 971-975, 29 April 2004

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RAT & MOUSE (In Vitro & Newborn) RETINA

Tracy L. Young and Constance L. Cepko

A role for ligand-gated ion channels in rod photoreceptor development

Neuron, Volume 41, Issue 6, Pages 867-879, 25 March 2004

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Takahiko Matsuda and Constance L. Cepko

Electroporation and RNA interference in the rodent retina in vivo and in vitro

PNAS (Proceedings of the National Academy of Sciences of the United States of America), Volume 101, Number 1, Pages 16-22, 6 January 2004

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Dezawa et al.

Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach

Micron Volume 33, Issue 1, Pages 1-6, 2002

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RAT   &   MOUSE LIVER


Nakagawa et al.

Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation

Genes & Development, Volume 22, Issue 1, Pages 37-49, 1 January 2008

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RAT  & MOUSE KIDNEY


Ma et al.

Local Actions of Endogenous Angiotensin II in Injured Glomeruli

Journal of the American Society of Nephrology, Volume 15, Issue 5, Pages 1268-1276, May 2004

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Yazawa et al.

Direct transfer of hepatocyte growth factor gene into kidney suppresses cyclosporin A nephrotoxicity in rats

Nephrology Dialysis Transplantation, Volume 19, Issue 4, Pages 812-816, April 2004

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RAT  &  MOUSE TESTIS & OVARY

Suzuki et al.

Effect of CaCl2 concentration on the rate of foreign gene transfer and expression by in vivo electroporation in the mouse ovary

International Journal of Molecular Medicine, Volume 12, Number 3, Pages 365-358, September 2003

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Hisano et al.

Methylation of CpG dinucleotides in the open reading frame of a testicular germ cell-specific intronless gene, Tact1/Actl7b, represses its expression in somatic cells

Nucleic Acids Research, Volume 31, Number 16, Pages 4797-4804, 15 August 2003

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Yomogida et al.

Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect

Biology of Reproduction, Volume 67, Issue 3, Pages 712-717, September 2002

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RAT & MOUSE MUSCLE

Takeshita et al.

Toll-Like Receptor Adaptor Molecules Enhance DNA-Raised Adaptive Immune Responses against Influenza and Tumors through Activation of Innate Immunity

Journal of Virology, Volume 80, Number 13, Pages 6218-6224, July 2006

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Saba et al.

Multivalent DNA vaccine protects mice against pulmonary infection caused by Pseudomonas aeruginosa

Vaccine, Volume 24, Issues 37-39, Pages 6240-6249, 11 September 2006

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Kitaguchi et al.

Immune deficiency enhances expression of recombinant human antibody in mice after nonviral in vivo gene transfer

International Journal of Molecular Medicine, Volume 16, Number 4, Pages 683-688, October 2005

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Umeda et al.

Skeletal muscle targeting in vivo electroporation-mediated HGF gene therapy of bleomycin-induced pulmonary fibrosis in mice

Laboratory Investigation, Volume 84, Issue 7, Pages 836-844, July 2004

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Yoshida et al.

Gene Therapy for Central Diabetes Insipidus: Effective Antidiuresis by Muscle-Targeted Gene Transfer

Endocrinology, Volume 145, Number 1, Pages 261-268, January 2004

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Xue et al.

Hepatocyte growth factor gene therapy accelerates regeneration in cirrhotic mouse livers after hepatectomy

Gut, Volume 52, Number 5, Pages 694-700, May 2003

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Kawai et al.

Ectopic Bone Formation by Human Bone Morphogenetic Protein-2 Gene Transfer to Skeletal Muscle Using Transcutaneous Electroporation

Human Gene Therapy, Volume 14, Number 16, Pages 1547-1556, November 2003

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Inoue et al.

Anti-Monocyte Chemoattractant Protein-1 Gene Therapy Limits Progression and Destabilization of Established Atherosclerosis in Apolipoprotein E-Knockout Mice

Circulation, Volume 106, Issue 21, Pages 2700-2706, 19 November 2002

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Ikeda et al.

Anti-monocyte chemoattractant protein-1 gene therapy attenuates pulmonary hypertension in rats

American Journal of Physiology – Heart and Circulatory Physiology, Volume 283, Issue 5, Pages H2021-H2028, November 2002

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Xue et al.

Attenuated acute liver injury in mice by naked hepatocyte growth factor gene transfer into skeletal muscle with electroporation

Gut, Volume 50, Number 4, Pages 558-562, April 2002

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Tanaka et al.

Gene Therapy for Mitochondrial Disease by Delivering Restriction Endonuclease SmaI into Mitochondria

Journal of Biomedical Science, Volume 9, Number 6, Pages 534-541, 2002

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Takada et al.

Infectivity-Enhancing Antibodies to Ebola Virus Glycoprotein

Journal of Virology, Volume 75, Number 5, Pages 2324-2330, March 2001

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Watanabe et al.

Protection Against Autoimmune Myocarditis by Gene Transfer of Interleukin-10 by Electroporation

Circulation, Volume 104, Issue 10, Pages 1098-1100, 4 September 2001

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Yasui et al.

Elevated gastrin secretion by in vivo gene electroporation in skeletal muscle

International Journal of Molecular Medicine, Volume 8, Number 5, Pages 489-494, November 2001

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RAT   &   MOUSE  JOINT

Saito et al.

Sonoporation mediated transduction of pDNA/siRNA into joint synovium in vivo

Journal of Orthopaedic Research, Volume 25, Issue 10, Pages 1308-1316, October 2007

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Inoue et al.

Electro-transfer of small interfering RNA ameliorated arthritis in rats

Biochemical and Biophysical Research Communications, Volume 336, Issue 3, Pages 903-908, 28 October 2005

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Ohashi et al.

Successful genetic transduction in vivo into synovium by means of electroporation

Biochemical and Biophysical Research Communications, Volume 293, Issue 5, Pages 1530-1535, 24 May 2002

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RAT   &   MOUSE   SKIN

Inoue et al.

Modulation of scratching behavior by silencing an endogenous cyclooxygenase-1 gene in the skin through the administration of siRNA

The Journal of Gene Medicine, Volume 9, Issue 11, Pages 994-1001, November 2007

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Maruyama et al.

Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

Epidermal Cells Methods and Protocols, Series: Methods in Molecular Biology, Volume 289, Pages 431-436, October 2004

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CHICK    EMBRYO    (In Ovo)

Towers et al.

Integration of growth and specification in chick wing digit-patterning

Nature, Volume 452, Number 7189, Pages 882-886, 17 April 2008

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Chang et al.

Bmp4 Is Essential for the Formation of the Vestibular Apparatus that Detects Angular Head Movements

PLoS Genetics, Volume 4, Issue 4, e1000050, 11 April 2008

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Sun et al.

Expression dynamics of the LIM-homeobox genes, Lhx1 and Lhx9, in the diencephalon during chick development

The International Journal of Developmental Biology, Volume 52, Number 1, Pages 33-41, 2008

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Ladher et al.

FGF8 initiates inner ear induction in chick and mouse

Genes and Development, Volume 19, Issue 5, Pages 603-613, March 2005

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Matsunaga et al.

RGM and its receptor neogenin regulate neuronal survival.

Nature Cell Biology, Volume 6, Issue 8, Pages 749-755, August 2004

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Nakamura et al.

Gain- and loss-of-function in chick embryos by electroporation

Mechanisms of Development, Volume 121, Issue 9, Pages 1137-1143, September 2004

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Wakamatsu et al.

Multiple roles of Sox2, an HMG-box transcription factor in avian neural crest development

Developmental Dynamics, Volume 229, Issue 1, Pages 74-86, January 2004

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Toyofuku et al.,

Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2

Genes & Development, Volume 18, Issue 4, Pages 435-447, 15 February 2004

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Tatsuya Katahira and Harukazu Nakamura

Gene silencing in chick embryos with a vector-based small interfering RNA system.

Development Growth & Differentiation, Volume 45, Issue 4, Pages 361-367, August 2003

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Sayaka Sugiyama and Harukazu Nakamura

The role of Grg4 in tectal laminar formation

Development, Volume 130, Issue 3, Pages 451-462, February 2003

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Sakiyama et al.

Tbx4-Fgf10 system controls lung bud formation during chicken embryonic development

Development, Volume 130, Issue 7, Pages 1225-1234, April 2003

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Takeuchi et al.

Tbx5 and Tbx4 trigger limb initiation through activation of the Wnt/Fgf signaling cascade

Development, Volume 130, Issue 12, Pages 2729-2739, June 2003

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Takeuchi at el.

Tbx5 specifies the left/right ventricles and ventricular septum position during cardiogenesis

Development, Volume 130, Issue 24, Pages 5953-5964, December 2003

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Jin et al.

Irx4-mediated regulation of Slit1 expression contributes to the definition of early axonal paths inside the retina

Development, Volume 130, Issue 6, Pages 1037-1048, March 2003

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C.-M. Amy Chen and Constance L. Cepko

The chicken RaxL gene plays a role in the initiation of photoreceptor differentiation

Development, Volume 129, Issue 23, Pages 5363-5375, December 2002

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Treubert-Zimmemann et al.

Targeting Axons to Specific Fiber Tracts In Vivo by Altering Cadherin Expression

The Journal of Neuroscience, Volume 22, Issue 17, Pages 7617-7626, 1 September 2002

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Oberg et al.

Efficient ectopic gene expression targeting chick mesoderm

Developmental Dynamics, Volume 224, Issue 3, Pages 291-302, July 2002

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Sato et al.

Inductive signal and tissue responsiveness defining the tectum and the cerebellum

Development, Volume 128, Issue 13, Pages 2461-2469, July 2001

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Nakamura et al.

Misexpression of genes in brain vesicles by in ovo electroporation

Development Growth & Differentiation, Volume 42, Issue 3, Page 199-201, June 2000

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Itasaki et al.

‘Shocking’ developments in chick embryology: electroporation and in ovo gene expression

Nature Cell Biology, Volume 1, Issue 8, Pages E203-E207, December 1999

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CHICK   EMBRYO  (New Culture)

Kinoshita et al.

Apical Accumulation of Rho in the Neural Plate Is Important for Neural Plate Cell Shape Change and Neural Tube Formation

Molecular Biology of the Cell, Volume 19, Issue 5, Pages 2289-2299, May 2008

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Iimura et al.

Dual mode of paraxial mesoderm formation during chick gastrulation

PNAS, Volume 104, Number 8, Pages 2744-2749, 20 February 2007

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Tadahiro Iimura and Olivier Pourquie

Collinear activation of Hoxb genes during gastrulation is linked to mesoderm cell ingression

Nature, Volume 442, Number 7102, Pages 568-571, 3 August 2006

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Wakamatsu et al.

Multiple roles of Sox2, an HMG-box transcription factor in avian neural crest development

Developmental Dynamics, Volume 229, Issue 1, Pages 74-86, January 2004

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Catherine E. Krull

A primer on using in ovo electroporation to analyze gene function

Developmental Dynamics, Volume 229, Issue 3, Pages 433-439, March 2004

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Toyofuku et al.,

Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2

Genes & Development, Volume 18, Issue 4, Pages 435-447, 15 February 2004

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Endo et al.

Bimodal functions of Notch-mediated signaling are involved in neural crest formation during avian ectoderm development

Development, Volume 129, Issue 4, Pages 863-873, February 2002

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Kobayashi et al.

Early subdivisions in the neural plate define distinct competence for inductive signals

Development, Volume 129, Issue 1, Pages 83-93, January 2002

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CHICK

Yamaguchi et al.

In-vivo gene transfer into newly hatched chick brain by electroporation

Neuroreport, Volume 18, Issue 8, Pages 735-739, 28 May 2007

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ZEBRAFISH RETINA

Thummel et al.,

Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina

Developmental Neurobiology, Volume 68, Issue 3, Pages 392-408, 15 February 2008

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ZEBRAFISH FIN

Thummel et al.,

Inhibition of zebrafish fin regeneration using in vivo electroporation of morpholinos against fgfr1 and msxb

Developmental Dynamics, Volume 235, Issue 2, Pages 336-346, February 2006

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Bai et al.,

Matrix metalloproteinase expression and function during fin regeneration in zebrafish: Analysis of MT1-MMP, MMP2 and TIMP2B

Matrix Biology, Volume 24, Issue 4, Pages 247-260, June 2005

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XENOPUS

Sasagawa et al.,

Improved mRNA Electroporation Method for Xenopus Neurula Embryos

Genesis, Volume 33, Issue 2, Pages 81-85, June 2002

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HONEY BEE

Takekazu Kunieda and Takeo Kubo

In vivo gene transfer into the adult honeybee brain by using electroporation

Biochemical and Biophysical Research Communications, Volume 318, Issue 1, Pages 25-31, 21 May 2004

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CRICKET

Shinmyo et al.

piggyBac-mediated somatic transformation of the two-spotted cricket, Gryllus bimaculatus

Development, Growth & Differentiation, Volume 46, Issue 4, Pages 343-349, August 2004

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PLANARIAN

Gonzalez-Estevez et al.,

Transgenic planarian lines obtained by electroporation using transposon-derived vectors and an eye-specific GFP marker

PNAS (Proceedings of the National Academy of Sciences of the United States of America), Volume 100, Number 24, Pages 14046-14051, 25 November 2003

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ADHERENT CELL

Usui et al.,

Expression Regulation of Hyaluronan Synthase in Corneal Endothelial Cells

Investigative Ophthalmology and Visual Science, Volume 41, Number 11, Pages 3261-3267, October 2000

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ES CELL

Yamada et al.

Electrical Stimulation Modulates Fate Determination of Differentiating Embryonic Stem Cells

Stem Cells, Volume 25, Number 3, Pages 562-570, March 2007

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ELECTROCHEMOTHERAPY

Shimizu et al.,

Electrochemotherapy for digital chondrosarcoma

Journal of Orthopaedic Science, Volume 8, Number 2, Pages 248-251, March 2003

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