Newsletter_2013-05

o  Step
3: reverses the polarity of the pulse and drives the pay-load in the opposite
direction

o Effectively
and uniquely, the first two steps of the NEPA21 electroporation process
ameliorate the destructive impact of the delivery of an electrical pulse.

o  Together,
these first two steps account for the increased Viability statistics achievable with the NEPA21. 

o  Voltage
Decay can also be programmed into the electroporation protocol – another
important differentiating feature of the NEPA21 compared with other
devices.  We have found this feature to contribute to both the enhanced
Viability and Transfection Efficiency obtainable with the NEPA21.

o  Compared
with other leading systems, it costs significantly less per electroporation to
run the NEPA21 system.  Less than Euro 2.00 per electroporation (if you
only use a cuvette once)

o  Previously,
if you wished to cover the full in vivo application range with our former
systems you had to purchase both the CUY21EDIT and CUY21SC
devices. 

For some laboratories this proved an expensive option.

o  But
now with the NEPA21 you get the

full in vivo application range in the
one device.  The NEPA21 combines in the one device BOTH the wider voltage
range of the CUY21EDIT and the enhanced sensitivity at lower voltages
of the CUY21SC.

o  Bearing
in mind the formula [V = IR]:  What this
means is that the target sample is first tested for R,
and then the delivered current is precisely controlled to obtain the
appropriate V.  The net effect of this is
that the electric fields delivered into the target are much more highly
controlled which, in turn, makes for more precise electroporation from one
experiment to the next and enhanced Viability and Transfection
Efficiencies.

o  Many
of the competing devices on the market are what we term voltage controlled
devices. What this means is that researchers (when they are
performing their experiments) are encouraged to almost exclusively focus on
voltage settings.  Such a ‘voltage only’ approach ignores the biological
fact that current varies greatly with the impedance of the target.  The
net effect is that many competing devices simply store charge on a capacitor
and then release the energy into the target without regard for or measuring the
resistance (impedance). 

o  For
many competing devices, the quality of the square wave decays during the
delivery of the pulse.  This is not the
case for the NEPA21.

 

Diploid, Normal

Normal, Immortalized

Acetabulum, Adherent

 

H1299

(LL/2)