LTM-1000 : Laser Thermal Microinjector
LTM-1000 : Laser Thermal Microinjector
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The unique micro-injector capable of PLANT CELL injection.
Compatible with glass micropipettes with 0.1μm tip
NOT available for sale in the USA, UK, Germany, France and Switzerland |
LTM-1000 Introduction Video Digest Version

Click on image to watch on YouTube |
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LTM-1000 Powerpoint Presentation
Click here for Application Note and Further Information
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Features
- Capable of producing high ejection pressures (+/- 1MPa per 1℃)
- Targets individual cell components
- Dramatically reduces cell death
- Multiple injections without clogging
- Rapid and convenient set-up & operation
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Stereoscope Microinjection Set-Up
Using LTM-1000 |
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APPLICATIONS |
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Microinject Materials: |
DNA, RNA, Protein, etc. |
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Capillary Tip Sizes: |
0.1μm or larger |
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Targets: |
Plant Cells, Fungal Cells, Mammalian Cells, Organelles |
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Injection into moss cells |
Stereoscope Microinjection Set-Up
Using LTM-1000 |
Laser Emission Wavelength |
980nm |
Laser Drive System |
ACC, Max. 2.5A, Continuously-Variable |
Optical Output |
1.3W |
Dimensions |
170W x 325D x 120H mm |
Weight |
4.1kg (includes laser head) |
Power |
100V- 240V, 50/60Hz |
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Number of injection into Plant Cells with a single capillary |
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Injection into the female gametophyte of plant |
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 LTM-1000(B).jpg) |

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Conventional Method(A) LTM-1000(B) |
Size Difference in Capillary Tips |
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Damaged cell using conventional method pictured on Left (A), No damage using LTM-1000 pictured on Right (B). |
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Injection of siRNA into the naked female gametophyte of Torenia
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Injection of plasmids into a nucleus of onion epidermal cells |
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Injection of antibody into plant cells |
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Injection into Budding Yeasts |
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Injection into Chloroplasts of Cyanidioschyzon merolae |
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Above information provided by: Dr. Tetsuya Higashiyama, Division of Biological Science, Graduate School of Science, Nagoya University
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“Laser-assisted Thermal-expansion Microinjection”
Patent Application Number (2004) 361448 |
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Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1 m (Knoblauch et al., Nature Biotech., 1999).
As the heat source, we chose a laser beam which enabled us to directly heat hydrophobic liquid containing laser absorber through the capillary glass and to precisely control the injection flow. |
*Please be aware that LTM-1000 can not be used for Arecaceae family due to the patent license.
LTM-1000 – Laser Thermal Microinjector |
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SW-25 – Hydrophobic ionic liquid, 1.5ml |
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WF-25 – Laser beam absorbent, 1.5ml |
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LTM-LH – Micropipette holder |
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We are pleased to announce that reference of the LTM-1000, Laser Thermal Microinjector, was published in Nature.(Nature, Volume 458, Number 7236, Pages 357-361, 19 March 2009)
Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells
It made the front cover of the Nature!
http://www.nature.com/nature/journal/v458/n7236/covers/