LTM-1000 : Laser Thermal Microinjector

The unique micro-injector capable of PLANT CELL injection.

Compatible with glass micropipettes with 0.1μm tip

NOT available for sale in the USA, UK, Germany, France and Switzerland

LTM-1000 Introduction Video Digest Version

Click on image to watch on YouTube

LTM-1000 Powerpoint Presentation

Click here for Application Note and Further Information

Features

Capable of producing high ejection pressures (+/- 1MPa per 1℃)
Targets individual cell components
Dramatically reduces cell death
Multiple injections without clogging
Rapid and convenient set-up & operation

Stereoscope Microinjection Set-Up
Using LTM-1000

APPLICATIONS

	Microinject Materials:	DNA, RNA, Protein, etc.
	Capillary Tip Sizes:	0.1μm or larger
	Targets:	Plant Cells, Fungal Cells, Mammalian Cells, Organelles

Injection into moss cells

Stereoscope Microinjection Set-Up
Using LTM-1000

SPECIFICATIONS

Laser Emission Wavelength	980nm
Laser Drive System	ACC, Max. 2.5A, Continuously-Variable
Optical Output	1.3W
Dimensions	170W x 325D x 120H mm
Weight	4.1kg （includes laser head）
Power	100V- 240Ｖ, 50/60Hz

Number of injection into Plant Cells with a single capillary

Injection into the female gametophyte of plant

Conventional Method(A) LTM-1000(B)

Size Difference in Capillary Tips

Damaged cell using conventional method pictured on Left (A), No damage using LTM-1000 pictured on Right (B).

Injection of siRNA into the naked female gametophyte of Torenia

Injection of plasmids into a nucleus of onion epidermal cells

Injection of antibody into plant cells

Injection into Budding Yeasts

Injection into Chloroplasts of Cyanidioschyzon merolae

Above information provided by: Dr. Tetsuya Higashiyama, Division of Biological Science, Graduate School of Science, Nagoya University

STRUCTURE

“Laser-assisted Thermal-expansion Microinjection”
Patent Application Number (2004) 361448

Stereoscope Microinjection Set-Up Using LTM-1000

Heat-induced expansion of liquid in a sealed capillary generates high pressure, which enables injection by capillaries with the tip diameter around 0.1

m (Knoblauch et al., Nature Biotech., 1999).
As the heat source, we chose a laser beam which enabled us to directly heat hydrophobic liquid containing laser absorber through the capillary glass and to precisely control the injection flow.

*Please be aware that LTM-1000 can not be used for Arecaceae family due to the patent license.

LTM-1000 – Laser Thermal Microinjector
SW-25 – Hydrophobic ionic liquid, 1.5ml
WF-25 – Laser beam absorbent, 1.5ml
LTM-LH – Micropipette holder

We are pleased to announce that reference of the LTM-1000, Laser Thermal Microinjector, was published in Nature.(Nature, Volume 458, Number 7236, Pages 357-361, 19 March 2009)
Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells
It made the front cover of the Nature!
http://www.nature.com/nature/journal/v458/n7236/covers/