SONIDEL Limited      :: sales@sonidel.com        :: tech-support@sonidel.com         :: Tel:  IRE, ++353 (0)1 4434358 UK, ++44 (0)20 3239 4904 USA, ++1 (617) 297 2435 .
Most Popular

NEPA21 Electroporator
ELEPO21 Electroporator
NEPA Porator
ECFG Porator
Transgenic Mouse Zygote Electroporation: NEPA21
Organoid Electroporation: NEPA21
Neuroscience & Genome Editing Publications
Hybridoma Production for Monoclonal Antibodies
Bacteria / Yeast Electroporation
SP100: Sonoporator
NEPA21: Illustrated  Applications
NEPA21: Publications by   Research Application
- Connection Cables
- CU700: Monitoring System
- CU902: Polarity Exchanger
- CUY21 EDIT Electroporator
- CUY21 EDIT-S Electroporator
- CUY21 SC Electroporator
- In Vivo, In vitro Electrodes
- Electroporation Cuvettes
- Cuvette Chamber & Stand   Holder
- Laser Thermal Microinjector
- Mechanical Vibration Units
- Ultrasounic BioMicroscope
- KTAC-4000: Sonoporator
- LF101: Cell Fusion Device
- LF201: Cell Fusion Device
- Electro Cell Fusion Electrodes
Electroporation and Electro   Cell Fusion Accessories
- Electroporators
- Publications/Protocols
- Electrodes by Product Codes

 

Product Search
Search by product code, name or application:


Full Product List
$catmenu
Electrode Search
Search for the most appropriate electrode by research application:

Publications/Protocols
- Sonoporation
- Electroporation
- ElectroCell Fusion
- What Is Sonoporation?

Electrode Recommendation
- Electrode Recommendations

Featured Applications
Sonoporation
SONIDEL SP100 and KTAC4000
Electroporation
CUY21 EDIT, CUY21 SC
Electro Cell Fusion
LF101 and LF201


Featured Device

0
Product Code: SONIDEL SP100

Desc:Sonoporator
Applic:(hover for application)Application:
  • Delivery of plasmids to cells and tissues for gene therapy-based applications and studies.
  • Delivery of nucleic acids such as siRNA, RNAi etc. to cells and tissues for studies on control of gene expression/gene therapy.
  • Delivery of cancer chemotherapeutic agents to impermeable target cells/tissues.
  • Delivery of agents to cells to study metabolic effects.


Featured Electrode



Product Code: CUY701P20L

Application:
Electrodes for Electroporation
Description:
20mm x 20mm platinum square electrode on tip of stick

Information
- How to order
- Contact us

Mouse/Rat – Skin

Electrode Recommendation:

To deliver genes into Mouse/Rat Skin by electroporation, we recommend:

–          CUY663-5×10 electrode

This electrode is a tweezers-type electrode.  One tweezers is a 5 x 10mm rectangular electrode and the other is three needles which look like a fork.

For your further information, some clients prefer to use the CUY650P electrode series for the same application.  The CUY650P series is also a tweezers type electrode but it incorporates disk electrodes.  With the CUY650P series, skin is picked up and simply sandwiched between the disk electrodes.

Technical Drawing: CUY650 series

 

Protocol Recommendation:

Kindly note Dr. Maruyama’s excellent know-how article:

–  Epidermis-targeted gene transfer using in vivo electroporation

In this article Dr. Maruyama uses the CUY21EDIT electroporator and CUY663B (currently catalogue number CUY663-5×10) electrode.

According to Dr. Maruyama’s paper, the required voltage is maximum 24V.  The appropriate electrode diameter depends on the size of the area into which the genes are to be transfected.  If one’s target area is small, the 1mm diameter (CUY650P1) electrode is appropriate.

Also of know are the following articles:

–  Chapter 27: Electroporation-Mediated Intradermal Delivery of DNA Vaccines in Nonhuman Primates

Modulation of scratching behavior by silencing an endogenous cyclooxygenase-1 gene in the skin through the administration of siRNA

 

Click here to view a summary of the unique advantages of the NEPA21 electroporation system and its novel multi-pulse EP Methodology


© Copyright 2024 Sonidel Limited. All Rights Reserved. XML Sitemap : User Sitemap