NEPA21_In_Vivo_Tumor_EP

NEPA21 In Vivo Tumor EP

Tumor EP with the NEPA21 system is very efficient and simple to achieve.

Please note the following NEPA21/CUY21 publications and EP Conditions.

The NEPA21 may be configured to deliver the follow single pulse EP Conditions:

Category Title Electrode Voltage P on P off No. of P
Mice/Rats
in vivo
Tumors		 Takei Y
Electroporation-mediated siRNA delivery into tumors.
Methods Mol Biol. 2014;1121:131-8.		  CUY663P3X6  70V  50ms 100ms 6 (3 & 3 with
polarity
exchange)
Shibata MA et al.
The endogenous soluble VEGF receptor-2 isoform suppresses lymph node metastasis in a mouse immunocompetent mammary cancer model.
BMC Med. 2010 Nov 3;8:69.		  CUY650P10  100V  20ms  8
Aung W et al.
Visualization of in vivo electroporation-mediated transgene expression in experimental tumors by optical and magnetic resonance imaging.
Gene Ther. 2009 Jul;16(7):830-9. Epub 2009 May 21.		  CUY663-5X8  50V  50ms 10 (5 & 5 with
polarity
exchange)
Shibata MA et al.,
Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model.
Cancer Gene Ther. 2008 Dec;15(12):776-86. Epub 2008 Jul 25.		  CUY650P10  100V  20ms  8
Kang JH
Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex.
Cancer Lett. 2008 Jul 8;265(2):281-8. Epub 2008 Mar 28.		  CUY653 50V/cm, 100V/cm,
250V/cm		 100ms  6
Takei Y et al.,
In vivo silencing of a molecular target by short interfering RNA electroporation: tumor vascularization correlates to delivery efficiency.
Mol Cancer Ther. 2008 Jan;7(1):211-21.		  CUY663P3X6  70V  50ms  100ms 6 (3 & 3 with
polarity
exchange

For NEPA21 customers only, please contact us to obtain NEPA21 multi-pulse EP Conditions.

Specifically, we direct you to the following publications:
– Electroporation-Mediated siRNA Delivery into Tumors
– In vivo silencing of a molecular target by short interfering RNA electroporation- tumor vascularization
– Visualization of in vivo electroporation-mediated transgene expression in experimental tumors by optical and magnetic resonance imaging

Also of specific interest is the following video of Tumor EP with the NEPA21:
– Tumor EP with the NEPA21

 

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