NEPA21 In Vitro Results
In Vitro – Cell Culture Electroporation with the NEPA21 System
With the NEPA21 system, one has a choice of two possible methods for In Vitro EP:
– with cuvettes and the CU500 Cuvette Chamber (for cells in suspension) and
– with the Adherent-Cell electrode, the CUY900-13-3-5 ( for cells cultured directly onto a plate and in adherence):
Neither choice requires additional expensive buffers
– Adherent-Cell Electroporation
– In Situ Gene Transfer of Adherent Cells by Electroporation
– Cuvette / Suspension-cell Electroporation
The following links to pages on our website, demonstrate results data obtained from client labs. for both Cuvette and Adherent-Cell electrode EP with the NEPA21 system.
No special buffers are required. Cost per electroporation with the cuvette method is E 2.00 per electroporation.
| List of Cells Transfected in Client labs.
– NEPA21 Cell Transfection Database – RESULTS
– For example, to search for results data on ‘neuron cells’, please open the link above and type ‘neur’ in the Find Field
– The search field is found by holding down the ‘Control’ key and pressing the ‘F’ key
– Then use the up/down arrows to move to the next item in the listing
– Then repeat the search procedure for the next target of interest: E.g., for ‘Epithelial Cells’, type ‘epithel’ in the Find Field
Example Results:
|
Cell Line
|
Description, Species
|
Characteristics,
etc.
|
V%
|
TE%
|
| Primary Cells: With Electroporation Cuvettes |
|
|
|
|
|
|
|
| PBMC |
Mouse Cerebral Cortex Neurons(Embryonic day 14) |
Primary, Adherent |
80%
|
70%
|
|
Mouse Hippocampal Neurons(Embryonic day 17) |
Primary, Adherent |
65%
|
70%
|
|
Mouse Hippocampal Neurons(Embryonic day 14) |
Primary, Adherent |
80%
|
60%
|
|
Mouse Cerebellar Granule Neurons |
Primary, Adherent |
91%
|
65%
|
|
Mouse Neural Progenitor Cells |
Primary, Adherent |
80%
|
60%
|
|
Mouse Cerebellar Granule Neurons |
Primary, Adherent |
91%
|
65%
|
|
Mouse DRG Neuronss |
Primary, Adherent |
70%
|
70%
|
| BMMC(BMMCs) |
Rat Cerebral Cortex Neurons(Embryonic day 16) |
Primary, Adherent |
70%
|
75%
|
|
Rat Hippocampal Neurons |
Primary, Adherent |
60%
|
80%
|
|
Rat Bubar Neurons(Embryonic day 15) |
Primary, Adherent |
80%
|
75%
|
|
|
|
|
|
| Cell LinesWith Electroporation Cuvettes |
|
|
| SK-N-SH |
Human Neuroblastoma cells |
Epithelial, Adherent |
95%
|
95%
|
| SH-SY5Y |
Human Neuroblastoma cells |
Adherent |
60%
|
90%
|
| SH-SY5Y |
Human Neuroblastoma cells |
Adherent |
70%
|
70%
|
| NB9 |
Human Neuroblastoma cells |
Adherent |
70%
|
70%
|
| NB69 |
Human Neuroblastoma cells |
Adherent |
95%
|
80%
|
| Neuro-2a |
Mouse Neuroblastoma cells |
Adherent |
90%
|
90%
|
|
|
|
|
|
| Using the CUY900-13-3-5 ElectrodeCells Lines in Adherent Status |
|
|
| SH-SY5Y |
Human Neuroblastoma cells |
Adherent |
90%
|
50%
|
| Neuro-2a |
Mouse Neuroblastoma cells |
Adherent |
80%
|
90%
|
|
|
|
|
|
| Stem Cells, ES Cells and iPS CellsWith Electroporation Cuvettes |
89%
|
76%
|
| TT2 |
Mouse iPS cell derived Neural Stem cells |
|
|
86%
|
|
Mouse Neural Stem cells |
|
90%
|
80%
|
|
Mouse Neural Stem cells |
|
80%
|
60%
|
|
Mouse NeurospheresThe cells were from E13.5 mouse brain: ganglionic eminence |
90%
|
75%
|
|
Mouse NeurospheresNeural Stem cells Derived from Mouse SVZ |
Excellent
|
Excellent
|
|
|
|
|
|
|
Cell
|
Characteristics
|
V%
|
TE%
|
| Using the CUY900-13-3-5 ElectrodePrimary Cells in Adherent Status |
|
| Mouse Cerebral Cortex Neurons(Embryonic day 15) (6 DIV) |
Primary, Adherent |
Excellent
|
Excellent
|
| Mouse Cerebral Cortex Neurons(Embryonic day 16) (2 DIV) |
Primary, Adherent |
70%
|
70%
|
| Mouse Cerebral Cortex Neurons(Embryonic day 16) (2 DIV) |
Primary, Adherent |
60%
|
65%
|
| Mouse Cerebral Cortex Neurons(Embryonic day 14) (5 DIV) |
Primary, Adherent |
95%
|
75%
|
| Mouse Hippocampal Neurons(Embryonic day 14) (4 DIV) |
Primary, Adherent |
60%
|
50%
|
| Mouse Hippocampal Neurons(Embryonic day 18) (2 DIV) |
Primary, Adherent |
85%
|
54%
|
| Mouse Neural Stem cells |
Primary, Adherent |
71%
|
71%
|
| Rat Cerebral Cortex Neurons(Embryonic day 17) (2 DIV) |
Primary, Adherent |
70%
|
60%
|
| Rat Hippocampal Neurons(Embryonic day 16) (2 DIV) |
Primary, Adherent |
70%
|
70%
|
| Rat Hippocampal Neurons(Postnatal day 7) (11 DIV) |
Primary, Adherent |
100%
|
50%
|
|
|
|
|
|
|
The above Cell Line results were achieved using our EC-002S Cuvettes (2mm gap)
For your information, No special buffers are required. Only our cuvettes.
For information:All clients who have purchases the NEPA21 system qualify for an automatic discount on further cuvette purchases.
EC-002S Cuvettes Prices:Standard Cuvette Price
| Pack |
No of Cuvettes |
Cost per Pack (Euro) |
Price per Cuvette (Euro) |
| 1 |
50 |
140.00 |
2.80 |
Client Discount Cuvette Prices**
| Pack |
No of Cuvettes |
Cost per Pack (Euro) |
Price per Cuvette (Euro) |
| 1 |
50 |
115.00 |
2.30 |
| 10 |
500 |
100.00 |
2.00 |
** Only clients who have purchased the NEPA21 device qualify for the Discounted Cuvette Prices
|
|
Images of Cells Transfected in Client labs.
– www.sonidel.com/NEPA21/NEPA21_Results/NEPA21_Cell_Images.pdf
Neuron Results from Client labs.
– www.sonidel.com/NEPA21/NEPA21_Results/NEPA21_neuron_data.pdf
Neurospheres – Neural Stem Cells
(Derived from Mouse SVZ, 24 hrs, 48 hrs and 72 Hrs after EP)
– Neurospheres – Neural Stem Cells Derived from Mouse SVZ
|