Cell Lines: With Electroporation Cuvettes

HeLa

Human Cervical Carcinoma cells

Viability: 95%

Transfection Efficiency: 95%

293 (HEK293)

Human Embryonic Kidney cells

Viability: 90%

Transfection Efficiency: 70%

293T (HEK293T)
Human Embryonic Kidney cells

Viability: 90%

Transfection Efficiency: 95%

TIG-3

Human Embryonic Lung Fibroblasts

Viability: 90%

Transfection Efficiency: 80%

TIG-7

Human Embryonic Lung Fibroblasts

Viability 89%

Transfection Efficiency 76%

MRC-5

Human Embryonic Lung Fibroblasts

Viability 85%

Transfection Efficiency 90%

SUSM-1

Human Fibroblasts

Viability 77%

Transfection Efficiency 71%

KMST-6

Human Fibroblasts

Viability 70%

Transfection Efficiency 60%

HT1080

Human Fibrosarcoma cells

Viability 93%

Transfection Efficiency 81%

MIA-PaCa-2

Human Pancreatic Carcinoma cells

Viability 80%

Transfection Efficiency 77%

HepG2

Human Hepatoma cells

Viability 88%

Transfection Efficiency 76%

HuH-7

Human Hepatoma cells

Viability: 70%

Transfection Efficiency: Excellent

H69

Human Small-Cell Lung Cancer cells

Viability: 90%

Transfection Efficiency: 85%

HSC-2

Human Squamous Carcinoma cells

Viability: 90-95%

Transfection Efficiency: 98%

HSC-3

Human Squamous Carcinoma cells

Viability: 90-95%

Transfection Efficiency: 98%

HSC-4

Human Squamous Carcinoma cells

Viability: 80%

Transfection Efficiency: 31%

Ca9-22

Human Squamous Carcinoma cells

Viability: 60%

Transfection Efficiency: 60%

HGF

Human Gingival Fibroblasts

Viability: Good

Transfection Efficiency: Good

BT-20

Human Breast Cancer cells

Viability: 70%

Transfection Efficiency: 80%

MCF-10A

Human Breast Cells

Viability: Good

Transfection Efficiency: Good

TE-1

Human Esophageal Carcinoma cells

Viability 85%

Transfection Efficiency 41%

TE-8

Human Esophageal Carcinoma cells

Viability 85%

Transfection Efficiency 40%

SKOV-3

Human Ovarian Carcinoma cells

Viability: 90%

Transfection Efficiency: 90%

OVCAR-3

Human Ovarian Carcinoma cells

Viability 90%

Transfection Efficiency 79%

RMG-1

Human Ovarian Clear Cell Adenocarcinoma

Viability 97%

Transfection Efficiency 67%

SK-N-SH

Human Neuroblastoma cells

Viability 95%

Transfection Efficiency 95%

SH-SY5Y

Human Neuroblastoma cells

*Left image: Lipofectamine LTX [ Viability:90％ Transfection Efficiency:10％]

Viability: 60%

Transfection Efficiency: 90%

KG-1-C

Human Oligodendroglial cells

Viability 85%

Transfection Efficiency 60%

A172

Human Glioblastoma cells

Viability: 85%

Transfection Efficiency: 70%

1321N1

Human Astrocytoma cells

Viability: 80%

Transfection Efficiency: 80%

HaCaT

Human Keratinocyte cells

Viability 40%

Transfection Efficiency 80%

CCD1079sk

Human Skin Fibroblasts

Viability 40%

Transfection Efficiency 53%

HTR-8/Svneo

Human Trophoblast cells

Viability 95%

Transfection Efficiency 67%

UT-7/TPO

Human Megakaryocytic cells

Viability: 51%

Transfection Efficiency: 46%

Jurkat

Human T-cell Leukemia cells

Viability: 90%

Transfection Efficiency: 85%

KG-1

Human Acute Myeloid Leukemia cells

Viability: 60%

Transfection Efficiency: 65%

Mutu 1

Human Burkitt Lymphoma cells

Viability: 87%

Transfection Efficiency: 91%

LCL

Human Lymphoblastoid cell

Viability: 53%

Transfection Efficiency: 43%

Ramos-BlueTM

Human Burkitt Lymphoma cells

Viability: 80%

Transfection Efficiency: 55%

K562

Human Erythroleukemia cells

Viability: 91%

Transfection Efficiency: 99%

THP-1

Human acute monocytic leukemia cells

Viability: 76%

Transfection Efficiency: 63%

NIH/3T3

Mouse Embryonic Fibroblasts

Viability: 74%

Transfection Efficiency: 81%

3T3-L1

Mouse Preadipocytes

Viability: 90%

Transfection Efficiency: 90%

MEF

Mouse Embryonic Fibroblasts

Viability: 75%

Transfection Efficiency: 70%

P19C6

Mouse Embryonic Carcinoma cells

Viability: 90%

Transfection Efficiency: 50%

L

Mouse Fibroblasts

Viability: 90%

Transfection Efficiency: 65%

MC3T3-E1

Mouse Osteoblastic cells

Viability 85%

Transfection Efficiency 75%

LLc1 (LL/2)

Mouse Lewis lung cell carcinoma 1 cells

Viability: 87%

Transfection Efficiency: 81%

C2C12

Mouse Myoblast cells

Viability: 80%

Transfection Efficiency: 70%

4T1

Mouse Breast Cancer cells

Viability: 90%

Transfection Efficiency: 95%

Colon-26

Mouse Colon Adenocarcinoma cells

Viability: 95%

Transfection Efficiency: 90%

ddy Mouse Endometrial cells

Viability: 60%

Transfection Efficiency: 80%

Neuro-2a

Mouse Neuroblastoma cells

Viability: 90%

Transfection Efficiency: 90%

BV-2

Mouse Microglial cells

Viability: 92%

Transfection Efficiency: 60%

WR19L

Mouse T-cell lymphoma cells

Viability: 92%

Transfection Efficiency: 60%

RAW264.7

Mouse Macrophage-like cells

Viability: 70%

Transfection Efficiency: 56%

MIN6

Mouse Pancreatic Beta cells

Viability 57%

Transfection Efficiency 71%

Mouse Pancreatic Islet Beta cells

Viability: 60%

Transfection Efficiency: 40%

XS106

Mouse Dendritic Cells

Viability 61%

Transfection Efficiency 45%

MC/9

Mouse Mast cells

Viability: 76%

Transfection Efficiency: 84%

BMBa

Mouse Bone marrow-derived basophils

Viability: 45%

Transfection Efficiency: 67%

ATDC5

Mouse Pre-chondrocytic cells

Blue:DAPI Green:GFP For ref. invitrogen:Lipofectamine LTX (with Plus Reagent) Transfection Efficiency:0.001%

Viability: 62%

Transfection Efficiency: 14%

PC12

Rat Adrenal Pheochromocytoma cells

Viability: 90%

Transfection Efficiency: 70%

H9c2

Rat Ventricular Myoblasts

Viability 71%

Transfection Efficiency 82%

REF

Rat Embryonic Fibroblasts

Viability 90%

Transfection Efficiency 99%

C6

Rat Glioma cells

Viability 80%

Transfection Efficiency 67%

RSC96

Rat Schwann cells

Viability: 70%

Transfection Efficiency: 85%

TtT/GF

Rat Anterior Pituitary cells

Viability: 65%

Transfection Efficiency: 83%

CHO

Chinese Hamster Ovary cells

Viability 74%

Transfection Efficiency 90%

CHO-K1

Chinese Hamster Ovary cells

Viability 95%

Transfection Efficiecncy 95%

DT40

Chicken B cells

Viability 38%

Transfection Efficiency 40%

MDCK

Madrin-Darby Canine

Viability: 90%

Transfection Efficiency: 95%

BFF

Bovine Fetal Fibroblasts

Viability: 78%

Transfection Efficiency: 72%

A6

Xenopus Kidney Epithelial cells

Viability: 90%

Transfection Efficiency: 60%

GTS9

Ginbuna Thymus cells

Viability: 50%

Transfection Efficiency: 30%

Primary Cells: With Electroporation Cuvettes

Primary HASM

Human Airway Smooth Muscle cells

Viability 90%

Transfection Efficiency 80%

Primary

Human Endometrial Stromal Cells

Viability 95%

Transfection Efficiency 90%

Primary

Human Uterine Cervical Fibroblast

Viability 65%

Transfection Efficiency 90%

Primary

Human T cells

The cells were cultured and stimulated with CD3 CD28 antibody for 66 hours before Electroporation

(the result of amaxa’s device: V about 20%  TE about 60%)

Viability: 58%

Transfection Efficiency: 90%

Primary

Mouse Cerebral Cortex Neurons

9 days after EP

Red: MAP2 Blue: GFAP Green: GFP The cells were from Embryonic Day 14 Mouse Cerebral Cortex

Viability 80%

Transfection Efficiency 70%

Primary

Mouse Neural Stem cells

Viability: Good

Transfection Efficiency: 37%

Primary

Mouse Microglia cells

Viability 65%

Transfection Efficiency 23%

Primary MEF

Mouse Embryonic Fibroblasts

Viability: 90%

Transfection Efficiency: 85%

Primary

Mouse Embryonic Skin Fibroblasts

Viability: 90%

Transfection Efficiency: 50%

Primary BMMC

Mouse Bone Marrow-Derived Mast Cells

Viability 64%

Transfection Efficiency 78%

Primary

Mouse Liver cells

Viability: 70%

Transfection Efficiency: 50%

Primary

Rat Cerebral Cortex Neurons

Viability: 64%

Transfection Efficiency: 78%

Primary

Rat Bulbar Neurons

7 days after EP / The cells were from Embryonic Day 15 Rat Bubblar Neurons

Viability 80%

Transfection Efficiency 75%

Primary

Rat Meningeal Fibroblasts

Viability: 90%

Transfection Efficiency: 95%

Primary

Rat Meningeal Fibroblasts

Viability 90%

Transfection Efficiency 95%

Primary

Rat Meningeal Fibroblasts

Viability: 90%

Transfection Efficiency: 95%

Primary OEC

Rat Olfactory Ensheathing Cells

5 days after electroporation – The cells were from Postnatal Week 3 – Electroporation: after being cultured for 2 weeks.

Viability 93%

Transfection Efficiency 46%

Primary

Chick Embryonic Fibroblasts

Viability: 80%

Transfection Efficiency: 90%

Primary

Chick Embryonic Cerebellar Granule Cells

Viability:

Transfection Efficiency: 35%

Primary

Human Mesenchymal Stem cells

Viability: 78%

Transfection Efficiency: 75%

Cord blood derived

Nucleofector (Lonza amaxa) result: Viability 20% Transfection Efficiency 20%

TS

Mouse Trophoblast Stem cells

Viability 59%

Transfection Efficiency 47%

Mouse TT2 ES cells

Viability: 55%

Transfection Efficiency: 55%

Mouse ES cells

Viability: 80%

Transfection Efficiency: 75%

Mouse iPS cells

Viability: 89%

Transfection Efficiency: 47%

Neurospheres

Neural Stem cells Derived from Mouse SVZ

Cell Lines in Adherence: With Adherent-Cell Electrode

SH-SY5Y

Human Neuroblastoma cells

In Adherence

Viability: 90%

Transfection Efficiency: 50%

EPC – Human Endothelial Progenitor Cells

In Adherence

Control

With Electroporation

3T3-L1 Mouse Preadipocytes

In Adherence

24 hours after electroporation

Electroporation: After 7 days of differentiation

NEPA21 Adherent Cell EP with CU600…………………………… CUY900-13-3-5 – Electrode

holder and CUY900-13-3-5 Electrode …………………………………for Adherent Cell EP

Viability: 90%

Transfection Efficiency: 70%

C6 Rat Glioma cells
In Adherence

Viability: 80%

Transfection Efficiency: 39%

Primary Cells in Adherent Status: With Adherent-Cell Elelctrode

Primary Mouse Neurons
In Adherence

Viability: Excellent

Transfection Efficiency: Excellent

Primary Mouse Neurons In Adherence

(High magnification)

Primary Mouse Neurons In Adherence

(High magnification)

Primary Mouse Hippocampal Neurons

In Adherence

Viability: 85%

Transfection Efficiency: 54%

Primary Mouse Glial cells

In Adherence

Viability: 80%

Transfection Efficiency: 50%

Primary Rat Neurons

In Adherence

Viability: 70%

Transfection Efficiency: 60%

24 hours after electroporation

The neurons were prepared from E17 rat cerebral cortex.

Electroporation: After 2 DIV (on 24-well plates)

Primary Rat Neurons

In Adherence

Viability: 100%

Transfection Efficiency: 50%

48 hours after electroporation

The neurons were prepared from P7 rat hippocampus.

Electroporation: After 11 DIV

Primary Rat Neurons

In Adherence

Viability: 100%

Transfection Efficiency: 50%

48 hours after electroporation

The neurons were prepared from P7 rat hippocampus.

Electroporation: After 11 DIV

Rat

Brain Slices

The neurons were prepared from P7 rat hippocampus.

Electroporation: After 11 DIV

Rat

Brain Slices

48 hours after electroporation

The neurons were prepared from P7 rat hippocampus.

Electroporation: After 11 DIV