SONIDEL SP100 Sonoporator

SONIDEL SP100^®

Sonoporation Platform

SONIDEL SP100^®– Optimised for enhanced ultrasound-mediated gene transfer

In Vitro, In Vivo, Ex Vivo, In Ovo transfection / nucleic acid transfer
Optimised pre-set ultrasound output programs for use with SONIDEL STK10^® Transfection Kit and SONIDEL MB101^® Microbubble
Precision engineered in Europe
User friendly
Self-contained robust ultrasound head
Ultrasound head compatible with direct insertion into tissue culture medium
A genuine cost break-through device
CE marked
Electrical and ultrasound safety comply fully with IEC safety standards

SONIDEL SP100^® with SONIDEL STK10^® Kit (including SONIDEL MB101^® Microbubble) Photographic Results

Plasmid DNA – pCMV-Luc			Minicircle DNA – MC07.CMV-luc

MB+DNA	MB+DNA+US	DNA+US	MB+DNA	MB+DNA+US	DNA+US
Plus MB101		No MB101	Plus MB101		No MB101
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Luciferase Expression using MB101 In Vivo (pCMV-luc and MC07.CMV-luc)


			Adherent Cells in Opticell ^®

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FEATURES

Output Frequency

Output frequency is fixed at precisely 1 MHz for optimised and reproducible penetration of ultrasound through tissue culture vessels and tissue-based targets.

Ultrasound Power/Density Options

Ultrasound power density/intensity options between 0 and 5 W/cm² with adjustments of 0.1 W/cm².

Duty Cycle Range

A wide range of duty cycles ranging from 5 – 100% in 5% increments and emitting at a pulse frequency of 100 Hz.

Automated Treatment Control Time

Automated control of treatment time that may be adjusted in seconds up to a treatment time of 90 seconds and thereafter in minutes up to a treatment time of 60 minutes.

Water-sealed Ultrasound head

The ultrasound head is water-sealed and compatible with operational immersion in liquid.

Pre-programmed Treatment Parameters

Supplied with 10 Operating Programs, 5 of which are pre-programmed to the appropriate treatment parameters to achieve optimal ultrasound-mediated transfection with the SONIDEL STK^® 10 Positive Control Transfection Kit. The other 5 programs may be adjusted to the specific conditions chosen by the operator

Ultrasound Dosage Feedback Control

The ultrasound head is equipped with a feedback control that automatically switches off the timer if contact with the target and transmission of ultrasound to the target is compromised. In this case the timer countdown mechanism will cease at the precise time contact was compromised and an audible alarm will sound.

Custom Features Available

Custom features may be supplied on demand.

SPECIFICATIONS

Frequency:	Continuous and pulsed ultrasound at an optimally pre-set precise frequency of 1 MHz.
Display:	Intensity in W/cm² (SATP*)
Contact control threshold:	65 %
Treatment time display and control buttons:	0 – 90 seconds and then switches to minutes (2-60), coupled to contact control. For enhanced operator control and feedback, the time display will stop counting down if adequate acoustic ultrasound contact with the target is compromised. This allows the operator to identify the precise time to which the target was exposed to ultrasound in the event of a failure in contact between the ultrasound head and the target
Ultrasound, continuous: Pulse frequency / duty cycle Power density/intensity (Output)	. 100 Hz / 100 % 0 – 5 W/cm², adjustable in 0.1 Wcm² increments
Ultrasound, pulsed: Pulse frequency / duty cycle Power density/intensity (Output)	. 100 Hz / 5-100 % in 5% increments 0 – 5 W/cm², adjustable in 0.1 Wcm² increments
Treatment head:1 MHz, Standard	Geometric surface area 1.5cm², Diameter 1.38cm, ERA 0.8cm², BNR* max. 6 type collimating, side panel radiation max. 10 mW/cm²
Mains adapter: Mains voltage Frequency Max, Power consumption	. 100 – 240 Volt 50/60 Hz 40 VA
Safety class:	*II according to IEC 60601-1
Dimensions:	220 x 200 x 195 mm
Weight:	1.7 kg
CE marking:	****
Safety standards:	IEC 60601-1 and IEC 60601-2-5
Environment conditions for Transport and Storage: Environment temperature Relative humidity Atmospheric pressure	..…………………………………………………………………………………… -10° till +50° C 10 till 100 % 500 till 1060 hPa
Environment conditions normal use: Environment temperature Relative humidity Atmospheric pressure	…………………………………………………………………………………………. 10° till 40° C 10 till 90 % (non condensing) 500 till 1060 hPa
* * * II *******	SATP = Spatial Average Temporal Peak (average pulse power) ERA = Effective Radiating Area, this is the effective radiating area of the treatment head BNR = Beam Nonuniformity Ratio, indicates the ratio between the peaks and the average value of the intensity in the sound beam. A low BNR excludes high, unwanted energy concentrations Safety class II (double insulated) According to European requirement 93/42 EEC

SONIDEL MB101^® Microbubble

Optimised for enhanced ultrasound-mediated gene transfer

Polymer-stabilized, lipid-based microbubble
Neutral surface charge and PEGylated for reduced non-specific binding
Sterile, liquid preparation
Ready to use with no requirement for specialised mixing devices
Stable for 6 months at 4⁰C
Optimized for use with the SONIDEL SP100 sonoporator
Designed specifically for ultrasound-mediated gene transfection / Nucleic Acid Transfer using in vivo and in vitro target systems
Non-invasive stimulation of gene transfer
Efficient gene/nucleic acid transfer
In vivo and in vitro applications
Compatible for use with plasmids and minicircle DNA
Minimal impact on cell/tissue viability
Supplied as part of the SONIDEL STK10 ultrasound transfection kit or as individual pack

SONIDEL SP100 Sonoporator