Protocol for in vivo electroporation into mouse and rat retina

NEPA21 / CUY21 Applications

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[Retina] Protocol for in vivo electroporation into mouse and rat retina

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(Figure 1)

Schematic illustration showing the in vivo electroporation method.

(1) DNA injection into subretinal space

(2) Electroporation

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Tweezer-type electrodes (3) are placed to hold the head of newborn (P0) rat or mouse (4).

In vivo electroporated rat retinae harvested at various developmental stages.

(A) Whole-mount preparation of rat retina in vivo electroporated with pCAG-GFP at P0 and harvested at P21. Pictures were taken from the scleral side.

(B) Rat retinae in vivo electroporated with pCAG-GFP at P0 were harvested at P2(d-f ), or P5(g-i), and cryosections were prepared.

Cepko laboratory, Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School; Takahiko Matsuda