Cultured Mouse Embryos and Large Tissue

Cultured Mouse and Rat Embryo and Large Tissue EP

Whole Mammalian Embryo EP:
There is a choice of two different electrodes:
–    CUY520P20 and
–    CUY650P7
(Click on links to view a technical drawing of the electrodes or similar electrodes in the series)

Please note the following article which explains the appropriate Methodology:
–    Electroporation into Cultured Mammalian Embryos
–    Methodology Summarised

Applicable Stages for Whole-Embryo Culture

NOTES
The volume of a bath can be changed by placing a small bath made of agarose gel in the bath.
Please refer to the Application Note: ‘Method to introduce genes into epithelial cells of the chicken embryonic stomach (proventriculus)’.

Embryonic Tissues (Kidney and Lung)
Result form client lab. for Embryonic Tissues (Kidney and Lung) using our CUY520P5 electrode (technical drawing).

 NEPA21 – Ex Vivo Publications
– Acute Inhibition of PI3K-PDK1-Akt Pathway Potentiates Insulin Secretion through Upregulation of Newcomer Granule Fusions in Pancreatic β-Cells
– Cortical excitatory neurons become protected from cell division during neurogenesis in an Rb family-dependent manner
– Electroporation-mediated gene transfer system applied to cultured CNS neurons
– Imaging of Insulin Exocytosis from Pancreatic Beta Cells
– In vivo ex vivo retina – ‘Chapter 19:Analysis of Gene Function in the Reina‘
– PSD95β regulates plasma membrane Ca(2+) pump localization at the photoreceptor synapse
– The Caudal Migratory Stream, The Caudal Migratory Stream: A Novel Migratory Stream ofInterneurons Derived from the Caudal Ganglionic Eminence in the Developing Mouse Forebrain

Small Tissue:
Electrode Recommendations:
–           CUY700 series and
–           CUY701 series
For smaller tissue of less than 0.5mm, we do not recommend the CUY520 series. The reason for this is practical; most clients want to minimize the required volume of DNA as much as possible and do not want to waste DNA.  For such applications we believe that the electroporation methods described in:
–  Electroporation-mediated gene transfer system applied to cultured CNS neurons
–  Early subdivisions in the neural plate define distinct comptence for inductive signals
are appropriate, that is, placing an electrode on the top of a tissue which is on another electrode.  A number of electrode option recommendations are appropriate:

Electrode Option 1:

–           CUY700P2E and CUY700P1L or CUY195P0.5

Electrode Option 2:

–          CUY700P2E and CUY614 with CUY615, one CUY613P1 or CUY611P3-1

See also Mouse and Rat Tissue/Brain Slice Recommendations