Test spacing 2
Cell Cultures (suspension electroporation) Human iPS cells |
Sayaka Deguchi, Tomohiro Shintani, Kazuo Harada,Toru Okamoto, Akinori Takemura, Kazumasa Hirata, Kousei Ito, Kazuo Takayama, Hiroyuki Mizuguchi In Vitro Model for a Drug Assessment of Cytochrome P450 Family 3 Subfamily A Member 4 Substrates Using Human Induced Pluripotent Stem Cells and Genome Editing Technology Hepatol. Commun. 05 May 2021. |
Tomoya Uchimura, Toshifumi Asano, Takao Nakata, Akitsu Hotta, Hidetoshi Sakurai A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs Cell Rep Med. 2021 Jun 4;2(6):100298. |
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Akihiro Yamashita, Hiroyuki Yoshitomi, Shunsuke Kihara, Junya Toguchida, Noriyuki Tsumaki Culture substrate-associated YAP inactivation underlies chondrogenic differentiation of human induced pluripotent stem cells Stem Cells Transl Med. 2021 Jan;10(1):115-127. |
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Takafumi Yumoto, Misaki Kimura, Ryota Nagatomo, Tsukika Sato, Shun Utsunomiya, Natsue Aoki, Motoji Kitaura, Koji Takahashi, Hiroshi Takemoto, Hirotaka Watanabe, Hideyuki Okano, Fumiaki Yoshida, Yosuke Nao, Taisuke Tomita Autism-associated variants of neuroligin 4X impair synaptogenic activity by various molecular mechanisms Mol Autism. 2020 Sep 1;11(1):68. |
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Thomas L Maurissen, Knut Woltjen Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation Nat Commun. 2020 Jun 8;11(1):2876. |
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Ben Sasaki, Shinji Uemoto, Yoshiya Kawaguchi Transient FOXO1 inhibition in pancreatic endoderm promotes the generation of NGN3+ endocrine precursors from human iPSCs Stem Cell Res. 2020 Apr;44:101754. |
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Peter Gee, Mandy S Y Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F Yang, Mio Iwasaki, Xiou H Wang, Matthew A Waller, Nanako Shirai, Yasuko O Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita, Kumiko A Iwabuchi, Masahiko Yasuda, Huaigeng Xu, Takeshi Noda, Jun Komano, Hidetoshi Sakurai, Naoto Inukai, Akitsu Hotta Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping Nat Commun. 2020 Mar 13;11(1):1334. |
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Eul Sik Jung, Zhejiu Quan, Mi-Yoon Chang, Wonjun Hong, Ji Hun Kim, Seung Hyun Kim, Seungkwon You, Dae-Sung Kim, Jiho Jang, Sang-Hun Lee,Hyongbum (Henry) Kim, Hoon Chul Kang Successful Correction of ALD Patient-derived iPSCs Using CRISPR/Cas9 bioRxiv February 25, 2020 |
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Morita Y, Andersen P, Hotta A, Tsukahara Y, Sasagawa N, Hayashida N, Koga C, Nishikawa M, Saga Y, Evans SM, Koshiba-Takeuchi K, Nishinakamura R, Yoshida Y, Kwon C, Takeuchi JK Sall1 transiently marks undifferentiated heart precursors and regulates their fate. J Mol Cell Cardiol. 2016 Mar;92:158-62. |
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Li HL, Gee P, Ishida K, Hotta A Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system. Methods. 2015 Oct 23. pii: S1046-2023(15)30133-X. |
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Hiromitsu Iizuka, Yuki Kagoya, Keisuke Kataoka, Akihide Yoshimi, Masashi Miyauchi, Kazuki Taoka, Keiki Kumano, Takashi Yamamoto, Akitsu Hotta, Shunya Arai, Mineo Kurokawa Targeted Gene Correction of RUNX1 in Induced Pluripotent Stem Cells Derived From Familial Platelet Disorder With Propensity to Myeloid Malignancy Restores Normal Megakaryopoiesis Exp Hematol, 43 (10), 849-57 Oct 2015 |
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Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Stem Cell Reports. 2015 Jan 13;4(1):143-54. |
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Reprogramming of iPS cells |
Yutong Jing, Yalan Zhou, Congxiang Wang, Jian Liu, Yonglong Guo, Shengru Mao, Hon Fai Chan, Shibo Tang, Jiansu Chen Establishment of a Non-Integrate iPS Cell Line CSUASOi002-A, From Urine-Derived Cells of a Female Patient With Macular Corneal Dystrophy Carrying Compound Heterozygous CHST6 Mutations Stem Cell Res, 41, 101598 Dec 2019 |
Human ES cells |
Namit Kumar, Yu-Hwai Tsai, Lei Chen, Anbo Zhou, Kushal K Banerjee, Madhurima Saxena, Sha Huang, Natalie H Toke, Jinchuan Xing, Ramesh A Shivdasani, Jason R Spence, Michael P Verzi The Lineage-Specific Transcription Factor CDX2 Navigates Dynamic Chromatin to Control Distinct Stages of Intestine Development Development, 146 (5) 2019 Mar 1 |
Karzbrun E, Kshirsagar A, Cohen SR, Hanna JH, Reiner O Human Brain Organoids on a Chip Reveal the Physics of Folding. Nat Phys. 2018 May;14(5):515-522. |
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Wang XL, Yu L, Ding Y, Guo XR, Yuan YH, Li DS Gene Manipulation of Human Embryonic Stem Cells by In Vitro-Synthesized mRNA for Gene Therapy. Curr Gene Ther. 2015;15(4):428-35. |
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Human Fetal Brain Organoids | H Hendriks D, Pagliaro A, Andreatta F, Ma Z, van Giessen J, MassaliniS, López-Iglesias C, van Son GJF, DeMartino J, Damen JMA, Zoutendijk I, Staliarova N, Bredenoord AL, Holstege FCP, Peters PJ, Margaritis T, Chuva de Sousa Lopes S, Wu W, Clevers H, Artegiani B. Human fetal brain self-organizes into long-term expanding organoids Cell . 2024 Jan 4:S0092-8674(23)01344-2. |
Murine Lacrimal Gland Organoids | Bannier-Hélaouët M, Geurts MH, Korving J, Begthel H, Clevers . Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids J V. 2023 Feb 3:(192).is Exp |
Patient Derived Airway Organoids | van der Vaart J, Böttinger L, Geurts MH, van de Wetering WJ, Knoops K, Sachs N, Begthel H, Korving J, Lopez-Iglesias C, Peters PJ, Eitan K, Gileles-Hillel A, Clevers H. Modelling of primary ciliary dyskinesia using patient-derived airway organoids EMBO Rep . 2021 Dec 6;22(12):e52058. |
Patient Derived Colorectal Tumor Organoids | Okamoto T, Natsume Y, Yamanaka H, Fukuda M, Yao R. A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids STAR Protoc. 2021 Sep 16;2(4):100780. |
Human Large Intestinal Organoids | Geurts MH, de Poel E, Pleguezuelos-Manzano C, Oka R, Carrillo L, Andersson-Rolf A, Boretto M, Brunsveld JE, van Boxtel R, Beekman JM, Clevers H. Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids Life Sci Alliance . 2021 Aug 9;4(10):e202000940. |
Human Breast Cancer Organoids | Dekkers JF, van Vliet EJ, Sachs N, Rosenbluth JM, Kopper O, Rebel HG, Wehrens EJ, Piani C, Visvader JE, Verissimo CS, Boj SF, Brugge JS, Clevers H, Rios AC. Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids Nat Protoc . 2021 Apr;16(4):1936-1965. |
Murine Lacrimal Gland Organoids | Bannier-Hélaouët M, Post Y, Korving J, Trani Bustos M, Gehart H, Begthel H, Bar-Ephraim YE, van der Vaart J, Kalmann R, Imhoff SM, Clevers H. Exploring the human lacrimal gland using organoids and single-cell sequencing Cell Stem Cell. 2021 Jul 1;28(7):1221-1232.e7 |
Human Gut Organoids | Hendriks D, Artegiani B, Hu H, Chuva de Sousa Lopes S, Clevers H. Establishment of human fetal hepatocyte organoids and CRISPR–Cas9-based gene knockin and knockout in organoid cultures from human liver Nat Protoc . 2021 Jan;16(1):182-217 |
Human Dissociated Large Intestinal Organoids |
Heinz MC, Oost KC, Snippert HJG. Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids STAR Protoc . 2020 Oct 8;1(3):100126. |
Human Dissociated Small Intestinal Organoids | |
Human Dissociated Intestinal Organoids |
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Human Dissociated organoids |
Maarten H Geurts, Shashank Gandhi, Matteo G Boretto, Ninouk Akkerman, Lucca L M Derks, Gijs van Son, Martina Celotti, Sarina Harshuk-Shabso, Flavia Peci, Harry Begthel, Delilah Hendriks, Paul Schürmann, Amanda Andersson-Rolf, Susana M Chuva de Sousa Lopes, Johan H van Es, Ruben van Boxtel, Hans Clevers One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids Nat Commun. 2023 Aug 17;14(1):4998. |
Rosemary Millen, Willem W B De Kort, Mandy Koomen, Gijs J F van Son, Roán Gobits, Bas Penning de Vries, Harry Begthel, Maurice Zandvliet, Patricia Doornaert, Cornelis P J Raaijmakers, Maarten H Geurts, Sjoerd G Elias, Robert J J van Es, Remco de Bree, Lot A Devriese, Stefan M Willems, Onno Kranenburg, Else Driehuis, Hans Clevers Patient-derived head and neck cancer organoids allow treatment stratification and serve as a tool for biomarker validation and identification Med. 2023 May 12;4(5):290-310.e12. |