APPLICATION

RAT & MOUSE

CHICK

ZEBRAFISH

XENOPUS

HONEY BEE

CRICKET

PLANARIAN

ADHERENT CELL

ES CELLS

ELECTROCHEMOTHERAPY

APPLICATION

PUBLICATION TITLE

ELECTRODE RECOMMENDATION

VOLTAGE

P ON

P OFF

No OF PULSES

RAT & MOUSE EMBRYO      (In Utero)

Sirt1 contributes critically to the redox-dependent fate of neural progenitors

Nature Cell Biology, Volume 10, Number 4, Pages 385-394, April 2008

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RECK modulates Notch signaling during cortical neurogenesis by regulating ADAM10 activity

Nature Neuroscience, Volume 10, Number 7, Pages 838-845, July 2007

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Radial Migration of Superficial Layer Cortical Neurons Controlled by Novel Ig Cell Adhesion Molecule MDGA1

The Journal of Neuroscience, Volume 26, Issue 17, Pages 4460-4464, 26 April 2006

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In vivo electroporation in the embryonic mouse central nervous system

Nature Protocols, Volume 1, Number 3, Pages 1552-1558, 2006

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Commissural neuron identity is specified by a homeodomain protein, Mbh1, that is directly downstream of Math1

Development, Volume 132, Issue 9, Pages 2147-2155, May 2005

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Neuronal generation, migration, and differentiation in the mouse hippocampal primoridium as revealed by enhanced green fluorescent protein gene transfer by means of in utero electroporation

The Journal of Comparative Neurology, Volume 483, Issue 3, Pages 329-340, 14 March 2005

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G Protein betagamma Subunits and AGS3 Control Spindle Orientation and Asymmetric Cell Fate of Cerebral Cortical Progenitors

Cell, Volume 122, Issue 1, Pages 119-131, 15 July 2005

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Birth-date-dependent segregation of the mouse cerebral cortical neurons in reaggregation cultures

European Journal of Neuroscience, Volume 22, Issue 2, Pages 331-342, July 2005

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Generation of Reelin-Positive Marginal Zone Cells from the Caudomedial Wall of Telencephalic Vesicles

The Journal of Neuroscience, Volume 24, Issue 9, Pages 2286-2295, 3 March 2004

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A NUDEL-dependent mechanism of neurofilament assembly regulates the integrity of CNS neurons

Nature Cell Biology, Volume 6, Number 7, Pages 595-608, July 2004

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Hes genes regulate size, shape and histogenesis of the nervous system by control of the timing of neural stem cell differentiation

Development, Volume 131, Issue 22, Pages 5539-5550, November 2004

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Mammalian BarH1 Confers Commissural Neuron Identity on Dorsal Cells in the Spinal Cord

The Journal of Neuroscience, Volume 23, Issue 6, Pages 1987-1991, 15 March 2003

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Kawauchi et al.

The in vivo roles of STEF/Tiam1, Rac1 and JNK in cortical neuronal migration

The EMBO Journal, Volume 22, Number 16, Pages 4190-4201, August 2003

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Layering defect in p35 deficiency is linked to improper neuronal-glial interaction in radial migration

Nature Neuroscience, Volume 6, Number 12, Pages 1284-1291, December 2003

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Multipolar Migration: The Third Mode of Radial Neuronal Migration in the Developing Cerebral Cortex

The Journal of Neuroscience, Volume 23, Issue 31, Pages 9996-10001, 5 November 2003

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Serine 732 Phosphorylation of FAK by Cdk5 Is Important for Microtubule Organization, Nuclear Movement, and Neuronal Migration

Cell, Volume 114, Issue 4, Pages 469-482, 22 August 2003

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Emx2 patterns the neocortex by regulating FGF positional signaling

Nature Neuroscience, Volume 6, Number 8, Pages 825-831, August 2003

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Roles of the Basic Helix-Loop-Helix Genes Hes1 and Hes5 in Expansion of Neural Stem Cells of the Developing Brain

The Journal of Biological Chemistry, Volume 276, Issue 32, Pages 30467-30474, 10 August 2001

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Efficient in utero gene transfer system to the developing mouse brain using electroporation: visualization of neuronal migration in the developing cortex

Neuroscience, Volume 103, Issue 4, Pages 865-872, 4 April 2001

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RAT & MOUSE CULTURED EMBRYO

Isolation and Characterization of Vasohibin-2 as a Homologue of VEGF-Inducible Endothelium-Derived Angiogenesis Inhibitor Vasohibin

Arteriosclerosis, Thrombosis, and Vascular Biology, Volume 26, Issue 5, Pages 1051-1057, May 2006

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Misrouting of mitral cell progenitors in the Pax6/small eyerat telencephalon

Development, Volume 131, Issue 4, Pages 787-796, February 2004

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Kawabata et al.

Electroporation-mediated gene transfer system applied to cultured CNS neurons

Neuroreport, Volume 15, Issue 6, Pages 971-975, 29 April 2004

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Pax6 regulates specification of ventral neurone subtypes in the hindbrain by establishing progenitor domains

Development, Volume 129, Issue 6, Pages 1327-1338, March 2002

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Manipulating gene expressions by electroporation in the developing brain of mammalian embryos

Differentiation, Volume 70, Issue 4-5, Pages 155-162, June 2002

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Gene Transfer into Cultured Mammalian Embryos by Electroporation

Methods, Volume 24, Issue 1, Pages 35-42, May 2001

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RAT  &  MOUSE BRAIN

Genetic manipulation of adult mouse neurogenic niches by in vivo electroporation

Nature Methods, Volume 5, Number 2, Pages 189-196, February 2008

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Locus-Specific Rescue of GluR1 NMDA Receptors in Mutant Mice Identifies the Brain Regions Important for Morphine Tolerance and Dependence

The Journal of Neuroscience, Volume 23, Issue 16, Pages 6529-6536, 23 July 2003

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Up-regulation of Protein-disulfide Isomerase in Response to Hypoxia/Brain Ischemia and Its Protective Effect against Apoptotic Cell Death

The Journal of Biological Chemistry, Volume 275, Issue 14, Pages 10388-10393, 7 April 2000

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RAT & MOUSE (Newborn) BRAIN

Plexin-A2 and its ligand, Sema6A, control nucleus-centrosome coupling in migrating granule cells

Nature Neuroscience, Volume 11, Number 4, Pages 440-449, April 2008

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Efficient In Vivo Electroporation of the Postnatal Rodent Forebrain

PLoS ONE, Volume 3, Issue 4, e1883, 2 April 2008

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Microtubule-based nuclear movement occursindependently of centrosome positioning in migrating neurons

PNAS, Volume 104, Number 41, Pages 16182-16187, October 9, 2007

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RAT  &  MOUSE BRAIN TISSUE

The Caudal Migratory Stream: A Novel Migratory Stream of Interneurons Derived from the Caudal Ganglionic Eminence in the Developing Mouse Forebrain

The Journal of Neuroscience, Volume 25, Issue 31, Pages 7268-7277, 3 August 2005

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Kawabata et al.

Electroporation-mediated gene transfer system applied to cultured CNS neurons

Neuroreport, Volume 15, Issue 6, Pages 971-975, 29 April 2004

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RAT & MOUSE (In Vitro & Newborn) RETINA

A role for ligand-gated ion channels in rod photoreceptor development

Neuron, Volume 41, Issue 6, Pages 867-879, 25 March 2004

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Electroporation and RNA interference in the rodent retina in vivo and in vitro

PNAS (Proceedings of the National Academy of Sciences of the United States of America), Volume 101, Number 1, Pages 16-22, 6 January 2004

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Gene transfer into retinal ganglion cells by in vivo electroporation: a new approach

Micron Volume 33, Issue 1, Pages 1-6, 2002

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RAT   &   MOUSE LIVER

Deubiquitylation of histone H2A activates transcriptional initiation via trans-histone cross-talk with H3K4 di- and trimethylation

Genes & Development, Volume 22, Issue 1, Pages 37-49, 1 January 2008

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RAT  & MOUSE KIDNEY

Ma et al.

Local Actions of Endogenous Angiotensin II in Injured Glomeruli

Journal of the American Society of Nephrology, Volume 15, Issue 5, Pages 1268-1276, May 2004

.

Yazawa et al.

Direct transfer of hepatocyte growth factor gene into kidney suppresses cyclosporin A nephrotoxicity in rats

Nephrology Dialysis Transplantation, Volume 19, Issue 4, Pages 812-816, April 2004

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RAT  &  MOUSE TESTIS & OVARY

Effect of CaCl₂ concentration on the rate of foreign gene transfer and expression by in vivo electroporation in the mouse ovary

International Journal of Molecular Medicine, Volume 12, Number 3, Pages 365-358, September 2003

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Methylation of CpG dinucleotides in the open reading frame of a testicular germ cell-specific intronless gene, Tact1/Actl7b, represses its expression in somatic cells

Nucleic Acids Research, Volume 31, Number 16, Pages 4797-4804, 15 August 2003

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Electroporated Transgene-Rescued Spermatogenesis in Infertile Mutant Mice with a Sertoli Cell Defect

Biology of Reproduction, Volume 67, Issue 3, Pages 712-717, September 2002

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RAT & MOUSE MUSCLE

Toll-Like Receptor Adaptor Molecules Enhance DNA-Raised Adaptive Immune Responses against Influenza and Tumors through Activation of Innate Immunity

Journal of Virology, Volume 80, Number 13, Pages 6218-6224, July 2006

.

Multivalent DNA vaccine protects mice against pulmonary infection caused by Pseudomonas aeruginosa

Vaccine, Volume 24, Issues 37-39, Pages 6240-6249, 11 September 2006

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Immune deficiency enhances expression of recombinant human antibody in mice after nonviral in vivo gene transfer

International Journal of Molecular Medicine, Volume 16, Number 4, Pages 683-688, October 2005

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Skeletal muscle targeting in vivo electroporation-mediated HGF gene therapy of bleomycin-induced pulmonary fibrosis in mice

Laboratory Investigation, Volume 84, Issue 7, Pages 836-844, July 2004

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Gene Therapy for Central Diabetes Insipidus: Effective Antidiuresis by Muscle-Targeted Gene Transfer

Endocrinology, Volume 145, Number 1, Pages 261-268, January 2004

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Hepatocyte growth factor gene therapy accelerates regeneration in cirrhotic mouse livers after hepatectomy

Gut, Volume 52, Number 5, Pages 694-700, May 2003

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Kawai et al.

Ectopic Bone Formation by Human Bone Morphogenetic Protein-2 Gene Transfer to Skeletal Muscle Using Transcutaneous Electroporation

Human Gene Therapy, Volume 14, Number 16, Pages 1547-1556, November 2003

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Anti-Monocyte Chemoattractant Protein-1 Gene Therapy Limits Progression and Destabilization of Established Atherosclerosis in Apolipoprotein E-Knockout Mice

Circulation, Volume 106, Issue 21, Pages 2700-2706, 19 November 2002

.

Anti-monocyte chemoattractant protein-1 gene therapy attenuates pulmonary hypertension in rats

American Journal of Physiology – Heart and Circulatory Physiology, Volume 283, Issue 5, Pages H2021-H2028, November 2002

.

Attenuated acute liver injury in mice by naked hepatocyte growth factor gene transfer into skeletal muscle with electroporation

Gut, Volume 50, Number 4, Pages 558-562, April 2002

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Tanaka et al.

Gene Therapy for Mitochondrial Disease by Delivering Restriction Endonuclease SmaI into Mitochondria

Journal of Biomedical Science, Volume 9, Number 6, Pages 534-541, 2002

Hamster

.

Infectivity-Enhancing Antibodies to Ebola Virus Glycoprotein

Journal of Virology, Volume 75, Number 5, Pages 2324-2330, March 2001

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Protection Against Autoimmune Myocarditis by Gene Transfer of Interleukin-10 by Electroporation

Circulation, Volume 104, Issue 10, Pages 1098-1100, 4 September 2001

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Elevated gastrin secretion by in vivo gene electroporation in skeletal muscle

International Journal of Molecular Medicine, Volume 8, Number 5, Pages 489-494, November 2001

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RAT   &   MOUSE  JOINT

Saito et al.

Sonoporation mediated transduction of pDNA/siRNA into joint synovium in vivo

Journal of Orthopaedic Research, Volume 25, Issue 10, Pages 1308-1316, October 2007

.

Electro-transfer of small interfering RNA ameliorated arthritis in rats

Biochemical and Biophysical Research Communications, Volume 336, Issue 3, Pages 903-908, 28 October 2005

.

Successful genetic transduction in vivo into synovium by means of electroporation

Biochemical and Biophysical Research Communications, Volume 293, Issue 5, Pages 1530-1535, 24 May 2002

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RAT   &   MOUSE   SKIN

Modulation of scratching behavior by silencing an endogenous cyclooxygenase-1 gene in the skin through the administration of siRNA

The Journal of Gene Medicine, Volume 9, Issue 11, Pages 994-1001, November 2007

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Epidermis-Targeted Gene Transfer Using In Vivo Electroporation

Epidermal Cells Methods and Protocols, Series: Methods in Molecular Biology, Volume 289, Pages 431-436, October 2004

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CHICK    EMBRYO    (In Ovo)

Integration of growth and specification in chick wing digit-patterning

Nature, Volume 452, Number 7189, Pages 882-886, 17 April 2008

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Bmp4 Is Essential for the Formation of the Vestibular Apparatus that Detects Angular Head Movements

PLoS Genetics, Volume 4, Issue 4, e1000050, 11 April 2008

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Expression dynamics of the LIM-homeobox genes, Lhx1 and Lhx9, in the diencephalon during chick development

The International Journal of Developmental Biology, Volume 52, Number 1, Pages 33-41, 2008

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FGF8 initiates inner ear induction in chick and mouse

Genes and Development, Volume 19, Issue 5, Pages 603-613, March 2005

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Matsunaga et al.

RGM and its receptor neogenin regulate neuronal survival.

Nature Cell Biology, Volume 6, Issue 8, Pages 749-755, August 2004

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Gain- and loss-of-function in chick embryos by electroporation

Mechanisms of Development, Volume 121, Issue 9, Pages 1137-1143, September 2004

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Multiple roles of Sox2, an HMG-box transcription factor in avian neural crest development

Developmental Dynamics, Volume 229, Issue 1, Pages 74-86, January 2004

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Toyofuku et al.,

Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2

Genes & Development, Volume 18, Issue 4, Pages 435-447, 15 February 2004

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Gene silencing in chick embryos with a vector-based small interfering RNA system.

Development Growth & Differentiation, Volume 45, Issue 4, Pages 361-367, August 2003

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The role of Grg4 in tectal laminar formation

Development, Volume 130, Issue 3, Pages 451-462, February 2003

.

Tbx4-Fgf10 system controls lung bud formation during chicken embryonic development

Development, Volume 130, Issue 7, Pages 1225-1234, April 2003

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Takeuchi et al.

Tbx5 and Tbx4 trigger limb initiation through activation of the Wnt/Fgf signaling cascade

Development, Volume 130, Issue 12, Pages 2729-2739, June 2003

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Tbx5 specifies the left/right ventricles and ventricular septum position during cardiogenesis

Development, Volume 130, Issue 24, Pages 5953-5964, December 2003

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Jin et al.

Irx4-mediated regulation of Slit1 expression contributes to the definition of early axonal paths inside the retina

Development, Volume 130, Issue 6, Pages 1037-1048, March 2003

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C.-M. Amy Chen and Constance L. Cepko

The chicken RaxL gene plays a role in the initiation of photoreceptor differentiation

Development, Volume 129, Issue 23, Pages 5363-5375, December 2002

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Targeting Axons to Specific Fiber Tracts In Vivo by Altering Cadherin Expression

The Journal of Neuroscience, Volume 22, Issue 17, Pages 7617-7626, 1 September 2002

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Efficient ectopic gene expression targeting chick mesoderm

Developmental Dynamics, Volume 224, Issue 3, Pages 291-302, July 2002

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Inductive signal and tissue responsiveness defining the tectum and the cerebellum

Development, Volume 128, Issue 13, Pages 2461-2469, July 2001

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Misexpression of genes in brain vesicles by in ovo electroporation

Development Growth & Differentiation, Volume 42, Issue 3, Page 199-201, June 2000

.

‘Shocking’ developments in chick embryology: electroporation and in ovo gene expression

Nature Cell Biology, Volume 1, Issue 8, Pages E203-E207, December 1999

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CHICK   EMBRYO  (New Culture)

Apical Accumulation of Rho in the Neural Plate Is Important for Neural Plate Cell Shape Change and Neural Tube Formation

Molecular Biology of the Cell, Volume 19, Issue 5, Pages 2289-2299, May 2008

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Dual mode of paraxial mesoderm formation during chick gastrulation

PNAS, Volume 104, Number 8, Pages 2744-2749, 20 February 2007

.

Collinear activation of Hoxb genes during gastrulation is linked to mesoderm cell ingression

Nature, Volume 442, Number 7102, Pages 568-571, 3 August 2006

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Multiple roles of Sox2, an HMG-box transcription factor in avian neural crest development

Developmental Dynamics, Volume 229, Issue 1, Pages 74-86, January 2004

.

A primer on using in ovo electroporation to analyze gene function

Developmental Dynamics, Volume 229, Issue 3, Pages 433-439, March 2004

.

Toyofuku et al.,

Dual roles of Sema6D in cardiac morphogenesis through region-specific association of its receptor, Plexin-A1, with off-track and vascular endothelial growth factor receptor type 2

Genes & Development, Volume 18, Issue 4, Pages 435-447, 15 February 2004

.

Bimodal functions of Notch-mediated signaling are involved in neural crest formation during avian ectoderm development

Development, Volume 129, Issue 4, Pages 863-873, February 2002

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Early subdivisions in the neural plate define distinct competence for inductive signals

Development, Volume 129, Issue 1, Pages 83-93, January 2002

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CHICK

In-vivo gene transfer into newly hatched chick brain by electroporation

Neuroreport, Volume 18, Issue 8, Pages 735-739, 28 May 2007

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ZEBRAFISH RETINA

Inhibition of Müller glial cell division blocks regeneration of the light-damaged zebrafish retina

Developmental Neurobiology, Volume 68, Issue 3, Pages 392-408, 15 February 2008

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ZEBRAFISH FIN

Inhibition of zebrafish fin regeneration using in vivo electroporation of morpholinos against fgfr1 and msxb

Developmental Dynamics, Volume 235, Issue 2, Pages 336-346, February 2006

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Matrix metalloproteinase expression and function during fin regeneration in zebrafish: Analysis of MT1-MMP, MMP2 and TIMP2B

Matrix Biology, Volume 24, Issue 4, Pages 247-260, June 2005

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XENOPUS

Sasagawa et al.,

Improved mRNA Electroporation Method for Xenopus Neurula Embryos

Genesis, Volume 33, Issue 2, Pages 81-85, June 2002

.

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HONEY BEE

In vivo gene transfer into the adult honeybee brain by using electroporation

Biochemical and Biophysical Research Communications, Volume 318, Issue 1, Pages 25-31, 21 May 2004

.

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CRICKET

piggyBac-mediated somatic transformation of the two-spotted cricket, Gryllus bimaculatus

Development, Growth & Differentiation, Volume 46, Issue 4, Pages 343-349, August 2004

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PLANARIAN

Transgenic planarian lines obtained by electroporation using transposon-derived vectors and an eye-specific GFP marker

PNAS (Proceedings of the National Academy of Sciences of the United States of America), Volume 100, Number 24, Pages 14046-14051, 25 November 2003

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ADHERENT CELL

Expression Regulation of Hyaluronan Synthase in Corneal Endothelial Cells

Investigative Ophthalmology and Visual Science, Volume 41, Number 11, Pages 3261-3267, October 2000

.

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ES CELL

Electrical Stimulation Modulates Fate Determination of Differentiating Embryonic Stem Cells

Stem Cells, Volume 25, Number 3, Pages 562-570, March 2007

.

ELECTROCHEMOTHERAPY

Electrochemotherapy for digital chondrosarcoma

Journal of Orthopaedic Science, Volume 8, Number 2, Pages 248-251, March 2003

.

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Please contact us for further information on Electrode Recommendations and Electroporation Protocols for any of the above applications