Controlling Electrofusion – LF101 & LF201
Recommended Protocol:
Kindly note the following article and Video Clip:
– ‘Timing controllable electrofusion device for aqueous dreplet-based microreactors’
The article details an electrofusion device (LF101 or LF201) for controlling the precise moment of fusion between droplets (β-galactosidase and fluorescein di-β-D-galactopyranoside (FDG)) by applying an electric field. This device allows:
– Accurate determination of the start of chemical/biological reactions
– Minimum contact of reactants with channel walls-eliminating surface absorption problems
– Easy fabrication
– Continuous observation of initiated reaction, and
– Enzymatic reaction using fluorescence microscopy was observed