NEPA21_In_Vivo_Tumor_EP
NEPA21 In Vivo Tumor EP
Tumor EP with the NEPA21 system is very efficient and simple to achieve.
Please note the following NEPA21/CUY21 publications and EP Conditions.
The NEPA21 may be configured to deliver the follow single pulse EP Conditions:
| Category | Title | Electrode | Voltage | P on | P off | No. of P |
| Mice/Rats in vivo Tumors |
Takei Y Electroporation-mediated siRNA delivery into tumors. Methods Mol Biol. 2014;1121:131-8. |
 CUY663P3X6 |  70V |  50ms | 100ms | 6 (3 & 3 with polarity exchange) |
| Shibata MA et al. The endogenous soluble VEGF receptor-2 isoform suppresses lymph node metastasis in a mouse immunocompetent mammary cancer model. BMC Med. 2010 Nov 3;8:69. |
 CUY650P10 |  100V |  20ms |  8 | ||
| Aung W et al. Visualization of in vivo electroporation-mediated transgene expression in experimental tumors by optical and magnetic resonance imaging. Gene Ther. 2009 Jul;16(7):830-9. Epub 2009 May 21. |
 CUY663-5X8 |  50V |  50ms | 10 (5 & 5 with polarity exchange) |
||
| Shibata MA et al., Combination therapy with short interfering RNA vectors against VEGF-C and VEGF-A suppresses lymph node and lung metastasis in a mouse immunocompetent mammary cancer model. Cancer Gene Ther. 2008 Dec;15(12):776-86. Epub 2008 Jul 25. |
 CUY650P10 |  100V |  20ms |  8 | ||
| Kang JH Effective delivery of DNA into tumor cells and tissues by electroporation of polymer-DNA complex. Cancer Lett. 2008 Jul 8;265(2):281-8. Epub 2008 Mar 28. |
 CUY653 | 50V/cm, 100V/cm, 250V/cm |
100ms | Â 6 | ||
| Takei Y et al., In vivo silencing of a molecular target by short interfering RNA electroporation:Â tumor vascularization correlates to delivery efficiency. Mol Cancer Ther. 2008 Jan;7(1):211-21. |
 CUY663P3X6 |  70V |  50ms |  100ms | 6 (3 & 3 with polarity exchange |
For NEPA21 customers only, please contact us to obtain NEPA21 multi-pulse EP Conditions.
Specifically, we direct you to the following publications:
– Electroporation-Mediated siRNA Delivery into Tumors
– In vivo silencing of a molecular target by short interfering RNA electroporation- tumor vascularization
– Visualization of in vivo electroporation-mediated transgene expression in experimental tumors by optical and magnetic resonance imaging
Also of specific interest is the following video of Tumor EP with the NEPA21:
– Tumor EP with the NEPA21