Plant Protoplast Fusion by Electro-Cell-Fusion and by Electroporation
Plant Protoplast Fusion
by
Electro Cell Fusion
Plant Protoplast Fusion may be performed by Electro Cell Fusion using our ECFG21 device (which has replaced the LF101 and LF201 devices).
Download: Plant Protoplast Fusion by Electro Cell Fusion Protocol (Cell Type: General Plant Protoplast and Celery Apium Graveolens L. Fusio) The stated methodologies illustrate the use of our LF201 or LF101 devices (now replaced by the ECFG21 device) and a chamber.
Electro Cell Fusion Electrode Recommendation: According to the protocol: – CUY501G4 (160ul) – CUY498-4 (2.0ml) – CUY498-10 (4.0ml) – are appropriate chambers. The number in brackets indicates the volume between electrodes. If one has a preference for the larger volume chamber electrode, then we recommend the CUY498-10 electrode.
Further electrode information: Our electrodes are compatible with the BTX ECM2001. However, they cannot be used with Eppendorf device.
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Re: Plant Seed Electroporation Link to further information on our webstie: Plant Seed Transfection by Electroporation The NEPA21 electroporator and the CUY495P15 electrode can be used for this protocol.
Please also note that you will also need to source the following items directly from these suppliers: – Dry Vacuum Pump (e.g. http://www.ulvac-kiko.com/en/pumps/da060d.html ) – Vacuum Desiccator (e.g. VM http://www.justis.as-1.co.jp/jus-tis/web/DirectSearch.aspx?shcode=1-5800-01 If the seed is hard, DNA cannot be transferred. Parameter setting for the following are available to our clients: – Protoplast (using our 2mm-gap Cuvette) – Plant Embryo EP (using our CUY520P5 electrode) – Isolated Chloroplasts (using our 2mm-gap Cuvette)
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Related Information:
Algae Transformation without cell-wall removal
ECFG21 device for Hybridoma Production/Nuclear Transfer