NEPA21_Algae-Transformation_without_cell-wall_removal

Algae Transformation without cell-wall removal

Chlamydomonas Reinhardtii

Diatom Phaeodactylum Tricornutum

26-fold higher transfection efficiency than Bio-Rad 

Two recent publications citing the NEPA21 Super Electroporator:

–     Rapid transformation of Chlamydomonas Reinhardtii without cell-wall removal

–     Highly Efficient Transformation of the Diatom Phaeodactylum Tricornutum by Multi-Pulse Electroporation

Pictures: Phaeodactylum tricornutum Provided courtesy of Dr. Kentaro Ifuku, Graduate School of Biostudies, Kyoto University and Dr. Jun-ichi Hotta, Graduate School of Science and Engineering, Yamagata University

Publication Information:
Chlamydomonas
–  Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal
–  Isolation and characterization of novel high-CO2-requiring mutants of Chlamydomonas reinhardtii
–  Isolation and characterization of mutants defective in the localization of LCIB, an essential factor for the carbonconcentrating mechanism in Chlamydomonas reinhardtii

Diatom
–  A stable and efficient nuclear transformation system for the diatom Chaetoceros gracili
–  Highly Efficient Transformation of the Diatom Phaeodactylum tricornutum by Multi-Pulse Electroporation
Euglena
–  Biochemical and physiological analyses of NADPH-dependent thioredoxin reductase isozymes in Euglena gracilis
–  Characterization and physiological role of two types of chloroplastic fructose-1,6-bisphosphatases in Euglena gracilis
–  Identification and characterization of cytosolic fructose-1,6-bisphosphatase in Euglena gracilis
–  Identification and functional analysis of peroxiredoxin isoforms in Euglena gracilis

Related links

– Plant Protoplast Fusion by Electro-Cell-Fusion and by Electroporation
– Plant Seed Electroporation (For Seeds of Rice Plant, Wheat and Barley, Silk Worm, Yeast, etc)
– Algae Transformation without cell-wall removal
– ECFG21 device for Hybridoma Production/Nuclear Transfer
– ECFG Illustrated Applications