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Product Code: CUY5100-100
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Application: Electrodes for Electro Cell Fusion |
Description: Chopstick Stainless Steel, needle electrode, 100µmφ, 5mm tip length, 2/pkg |
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Novel 2-Step Pulse Regime Electroporation System for In Vivo and In Vitro Applicaitons – including cells in adherence and cells in suspension
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07 March 2011
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Novel 2-Step Pulse Regime Electroporation System for In Vivo and In Vitro (including cells in adherence and cells in suspension) Applications
NEPA21
The NEPA21 Electroporator is based on the same platform and core technology that made the CUY21EDIT and CUY21SC devices leaders in their class. The major innovations in the NEPA21system are its novel 2-Step Pulse regime and polarity exchange capability.
2-STEP PULSE REGIME AND POLARITY EXCHANGE:
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The 2-Step pulse regime with voltage decay and polarity exchange facilitates more highly controlled electroporation pulse delivery.
This results in higher transfection efficiency and higher cell viability.
Step 1 |
Pore-forming Pulse Mode:
High Voltage, Short Duration, Single/Multiple Pulse(s), Voltage Decay
This poration pulse is for forming pores in the cell membrane with minimum residual cell damage
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Step 2-1
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Transfer Pulse Mode:
Low Voltage, Long Duration, Multiple Pulses, Voltage Decay.
This transfer pulse is for delivering the target molecules (DNA, RNA, etc.) into cells with minimum residual cell damage
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Step 2-2
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Polarity Exchanged Transfer Pulse:
This can improve the transfection efficiency and again, minimise residual cell damage
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Conscious that excellent transfection efficiency and cell viability is achievable using the Single-Step pulse regimes delivered by the CUY21SC and CUY21EDIT systems and that this performance is still the market-leading benchmark performance standard, we have ensured that the NEPA21 device can also deliver the Single-Step pulse regimes of those systems.
The effect of this innovation is that the NEPA21 system not only incorporates the full In Vivo, In Utero, In Ovo and Ex Vivo application ranges of the original CUY21EDIT and CUY21SC devices, but it also includes a completely new novel In Vitro (Suspended Cells and Adherent Cell)Â application range.
Moreover, the NEPA21 system also affords the user considerably lower operating-cost advantages for Suspended Cell and Adherent Cell (in commercially available cell culture plates) applications as the system is designed such that there is no requirement for expensive specialised reagents.
DISPOSABLE KIT COMPARISONS:
NEPA21 (Nepa Gene) |
Device: N (Company L) |
Device: N (Company I) |
NEPA ElectroporationCuvette (50 pieces)
Euro 90.00
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N Transfection Kit (25 tips)
Euro 375.00
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One Electroporation
Euro 1.80
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One Electroporation
$16.00
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One Electroporation
Euro 15.00
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*Euro Price |
For many of our clients the NEPA21′s lower running cost is a major factor in the decision to purchase the device. |
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APPLICATIONS CATEGORIES AND EXAMPLES:
APPLICATION |
CATEGORIES         and        EXAMPLES |
IN VITRO
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Cell Culture |
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DIFFICULT TO TRANSFECT CELLS:
–Â Â Â Primary Cells
o   BMMC Primary Mouse Bone Marrow-Derived
Mast cells
o   Primary Mouse Neurons, Cerebral Cortex
o   Primary Rat Bulbar Nerve Cells
o   HASM  Primary Human Airway Smooth
Muscle cells
o   Primary Human Endometrial Stromal Cells
–Â Â Â Neurons
–Â Â Â Stem Cells
–Â Â Â Blood Cells
–Â Â Â Immune Cells
–Â Â Â Etc
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NEPA21 Cell Image Results
Cell Transfection Data
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Adherent |
PRIMARY CELLS:
–Â Â Â Cultured Primary Neurons in adherence
–Â Â Â MOUSE EMBRYO (E15) CEREBRAL CORTEX
Adherent Cell Results
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Tissues/Organs (Ex Vivo) |
TISSUES/ORGANS:
–Â Â Â Tissue Slice
–Â Â Â Brain Slices
–Â Â Â Organ
–Â Â Â Whole Embryo
–Â Â Â Etc
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IN VIVO |
In Vivo: Mouse/Rat |
MOUSE/RAT:
–Â Â Â Brain
–Â Â Â Eye
–Â Â Â Muscle
–Â Â Â Skin
–Â Â Â Kidney
–Â Â Â Liver
–Â Â Â Tests
–Â Â Â Embryo
–Â Â Â Etc
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In Utero: Mouse/Rat Embryo |
MOUSE/RAT EMBRYO:
–Â Â Â Cerebral
–Â Â Â Cortex
–Â Â Â Hippocampus
–Â Â Â Spinal Cord
–Â Â Â Etc
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In Ovo: Chick Embryo |
CHICK EMBRYO:
–Â Â Â Neural Tube
–Â Â Â Limb Bud
–Â Â Â Digestive Organ
–Â Â Â Etc
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